Rabbit SCOP Polyclonal Antibody | anti-SCOP antibody

SCOP Antibody

Cat. Num. AAA9608923

• Gene Names: PHLPP1; SCOP; PHLPP; PPM3A; PLEKHE1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: SCOP; Polyclonal Antibody; SCOP Antibody; hSCOP; KIAA0606; PH domain and leucine rich repeat protein phosphatase; PH domain leucine rich repeat containing protein phosphatase; PH domain leucine rich repeat protein phosphatase; PH domain leucine-rich repeat-containing protein phosphatase 1; PH domain-containing family E member 1; PHLP1_HUMAN; PHLPP; Phlpp1; Pleckstrin homology domain containing family E (with leucine rich repeats) member 1; Pleckstrin homology domain containing family E protein 1; Pleckstrin homology domain-containing family E member 1; PLEKHE 1 protein; PLEKHE1; SCN circadian oscillatory protein; Suprachiasmatic nucleus circadian oscillatory protein; Suprachiasmatic nucleus circadian protein; anti-SCOP antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SCOP antibody detects endogenous levels of total SCOP
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 1717

• Applicable Applications for anti-SCOP antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1000-3000
• Immunogen: A synthesized peptide derived from human SCOP
• Subcellular Location: Cytoplasm. Membrane. Nucleus. In colorectal cancer tissue, expression is concentrated at the lateral membrane of epithelial cells.
• Tissue Specificity: In colorectal cancer tissue, expression is highest in the surface epithelium of normal colonic mucosa adjacent to the cancer tissue but is largely excluded from the crypt bases. Expression is lost or significantly decreased in 78% of tested tumors (at protein level). Ubiquitously expressed in non-cancerous tissues.
• Predicted Cross Reactivity: Pig, Horse, Rabbit
• Similarity: Pig (89%), Horse (89%), Rabbit (89%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis SCOP using COLO205 whole cell lysates)

Related Product Information for anti-SCOP antibody

Function: Protein phosphatase involved in regulation of Akt and PKC signaling. Mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family; specifically acts on 'Ser-473' of AKT2 and AKT3, 'Ser-660' of PRKCB and 'Ser-657' of PRKCA (PubMed:15808505, PubMed:17386267, PubMed:18162466). Isoform 2 seems to have a major role in regulating Akt signaling in hippocampal neurons (By similarity). Akt regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro-and antiapoptotic genes. Dephosphorylation of 'Ser-473' of Akt triggers apoptosis and suppression of tumor growth. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation (PubMed:18162466). Dephosphorylates STK4 on 'Thr-387' leading to STK4 activation and apoptosis (PubMed:20513427). Dephosphorylates RPS6KB1 and is involved in regulation of cap-dependent translation (PubMed:21986499). Inhibits cancer cell proliferation and may act as a tumor suppressor (PubMed:19079341). Dephosphorylates RAF1 inhibiting its kinase activity (PubMed:24530606). May act as a negative regulator of K-Ras signaling in membrane rafts (By similarity). Involved in the hippocampus-dependent long-term memory formation (By similarity). Involved in circadian control by regulating the consolidation of circadian periodicity after resetting (By similarity). Involved in development and function of regulatory T-cells (By similarity).
Subunit Structure: Interacts with the nucleotide free form of K-Ras (KRAS) via its LRR repeats (By similarity). Interacts with AKT2, AKT3, PRKCB isoform beta-II, STK4, RPS6KB1, RAF1 (PubMed:17386267, PubMed:18162466, PubMed:19732725, PubMed:21986499, PubMed:24530606). Isoform 1 (predominantly) and isoform 2 interact with BRAP (PubMed:25820252). Interacts with FKBP5; FKBP5 acts as a scaffold for PHLPP1 and Akt (PubMed:19732725). Interacts with SCRIB; SCRIB acts as a scaffold for PHLPP1 and Akt (PubMed:21701506). Interacts with SLC9A3R1; SLC9A3R1 scaffolds a heterotrimeric complex with PTEN at the plasma membrane (PubMed:21804599). Interacts with WDR48 and USP12 (PubMed:24145035).
Similarity: The PH domain is required for interaction with PRKCB and its dephosphorylation.

References

Dai X, Zhang N, Cheng Y, Yang T, Chen Y, Liu Z, Wang Z, Yang C, Jiang Y; Journal: Carcinogenesis. RNA-binding Protein Trinucleotide repeat-containing 6A Regulates the Formation of Circular RNA 0006916, with Important Functions in Lung Cancer Cells.

NCBI and Uniprot Product Information

• NCBI GI #: 291219891
• NCBI GeneID: 23239
• NCBI Accession #: NP_919431.2
• NCBI GenBank Nucleotide #: NM_194449.3
• UniProt Accession #: O60346; Q641Q7; Q6P4C4; Q6PJI6; Q86TN6; Q96FK2; Q9NUY1; A1A4F5
• Molecular Weight: Observed: 184 kDa; Predicted: 185 kDa
• NCBI Official Full Name: PH domain leucine-rich repeat-containing protein phosphatase 1
• NCBI Official Synonym Full Names: PH domain and leucine rich repeat protein phosphatase 1
• NCBI Official Symbol: PHLPP1
• NCBI Official Synonym Symbols: SCOP; PHLPP; PPM3A; PLEKHE1
• NCBI Protein Information: PH domain leucine-rich repeat-containing protein phosphatase 1
• UniProt Protein Name: PH domain leucine-rich repeat-containing protein phosphatase 1
• Protein Family: Scopoletin glucosyltransferase
• UniProt Gene Name: PHLPP1
• UniProt Synonym Gene Names: KIAA0606; PHLPP; PLEKHE1; SCOP; PH domain-containing family E member 1; hSCOP

NCBI Description

This gene encodes a member of the serine/threonine phosphatase family. The encoded protein promotes apoptosis by dephosphorylating and inactivating the serine/threonine kinase Akt, and functions as a tumor suppressor in multiple types of cancer. Increased expression of this gene may also play a role in obesity and type 2 diabetes by interfering with Akt-mediated insulin signaling. [provided by RefSeq, Dec 2011]

Uniprot Description

Protein phosphatase involved in regulation of Akt and PKC signaling. Mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family; specifically acts on 'Ser-473' of AKT2 and AKT3, 'Ser-660' of PRKCB and 'Ser-657' of PRKCA (PubMed:15808505, PubMed:17386267, PubMed:18162466). Isoform 2 seems to have a major role in regulating Akt signaling in hippocampal neurons (). Akt regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of Akt triggers apoptosis and suppression of tumor growth. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation (PubMed:18162466). Dephosphorylates STK4 on 'Thr-387' leading to STK4 activation and apoptosis (PubMed:20513427). Dephosphorylates RPS6KB1 and is involved in regulation of cap-dependent translation (PubMed:21986499). Inhibits cancer cell proliferation and may act as a tumor suppressor (PubMed:19079341). Dephosphorylates RAF1 inhibiting its kinase activity (PubMed:24530606). May act as a negative regulator of K-Ras signaling in membrane rafts (). Involved in the hippocampus-dependent long-term memory formation (). Involved in circadian control by regulating the consolidation of circadian periodicity after resetting (). Involved in development and function of regulatory T-cells ().