Rabbit anti-Human SCARB1 Polyclonal Antibody | anti-SCARB1 antibody

SCARB1, NT (SCARB1, CD36L1, CLA1, Scavenger receptor class B member 1, CD36 and LIMPII analogous 1, CD36 antigen-like 1, Collagen type I receptor, thrombospondin receptor-like 1, SR-BI, CD36) (PE)

Cat. Num. AAA6345238

• Gene Names: SCARB1; CLA1; SRB1; CLA-1; SR-BI; CD36L1; HDLQTL6
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SCARB1; Polyclonal Antibody; NT (SCARB1; CD36L1; CLA1; Scavenger receptor class B member 1; CD36 and LIMPII analogous 1; CD36 antigen-like 1; Collagen type I receptor; thrombospondin receptor-like 1; SR-BI; CD36) (PE); CD36; anti-SCARB1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-SCARB1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SCARB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 72-101 amino acids from the N-terminal region of human SCARB1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(SCARB1 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the SCARB1 antibody detected the SCARB1 protein (arrow).)

Related Product Information for anti-SCARB1 antibody

Receptor for different ligands such as phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and apoptotic cells. Probable receptor for HDL, located in particular region of the plasma membrane, called caveolae. Facilitates the flux of free and esterified cholesterol between the cell surface and extracellular donors and acceptors, such as HDL and to a lesser extent, apoB-containing lipoproteins and modified lipoproteins. Probably involved in the phagocytosis of apoptotic cells, via its phosphatidylserine binding activity. Receptor for hepatitis C virus glycoprotein E2. Binding between SCARB1 and E2 was found to be independent of the genotype of the viral isolate. Plays an important role in the uptake of HDL cholesteryl ester (By similarity).

Product Categories/Family for anti-SCARB1 antibody

Antibodies; Receptors; Scavenger

References

Kolmakova, A., et al. Endocrinology 151(11):5519-5527(2010). Shimada, M., et al. Hum. Genet. 128(4):433-441(2010). Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Teslovich, T.M., et al. Nature 466(7307):707-713(2010). Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 132566684
• NCBI GeneID: 949
• NCBI Accession #: NP_005496.4
• NCBI GenBank Nucleotide #: NM_005505.4
• UniProt Accession #: Q8WTV0; Q14016; Q52LZ5; Q6KFX4; F8W8N0
• Molecular Weight: 56,170 Da
• NCBI Official Full Name: scavenger receptor class B member 1 isoform 1
• NCBI Official Synonym Full Names: scavenger receptor class B, member 1
• NCBI Official Symbol: SCARB1
• NCBI Official Synonym Symbols: CLA1; SRB1; CLA-1; SR-BI; CD36L1; HDLQTL6
• NCBI Protein Information: scavenger receptor class B member 1; CD36 and LIMPII analogous 1; CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 1; scavenger receptor class B type III
• UniProt Protein Name: Scavenger receptor class B member 1
• Protein Family: Scavenger receptor
• UniProt Gene Name: SCARB1
• UniProt Synonym Gene Names: CD36L1; CLA1; SRB1; CLA-1
• UniProt Entry Name: SCRB1_HUMAN

NCBI Description

The protein encoded by this gene is a plasma membrane receptor for high density lipoprotein cholesterol (HDL). The encoded protein mediates cholesterol transfer to and from HDL. In addition, this protein is a receptor for hepatitis C virus glycoprotein E2. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]

Uniprot Description

SCARB1: Receptor for different ligands such as phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and apoptotic cells. Probable receptor for HDL, located in particular region of the plasma membrane, called caveolae. Facilitates the flux of free and esterified cholesterol between the cell surface and extracellular donors and acceptors, such as HDL and to a lesser extent, apoB-containing lipoproteins and modified lipoproteins. Probably involved in the phagocytosis of apoptotic cells, via its phosphatidylserine binding activity. Receptor for hepatitis C virus glycoprotein E2. Binding between SCARB1 and E2 was found to be independent of the genotype of the viral isolate. Plays an important role in the uptake of HDL cholesteryl ester. Belongs to the CD36 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: cell surface; lysosomal membrane; microvillus membrane; integral to plasma membrane; plasma membrane; caveola

Molecular Function: apolipoprotein A-I binding; protein binding; protein homodimerization activity; lipopolysaccharide binding; transporter activity; phosphatidylserine binding; low-density lipoprotein binding; lipopolysaccharide receptor activity; apolipoprotein binding; high-density lipoprotein binding; phosphatidylinositol binding

Biological Process: receptor-mediated endocytosis; wound healing; blood vessel endothelial cell migration; viral reproduction; positive regulation of nitric-oxide synthase activity; lipoprotein metabolic process; cholesterol efflux; androgen biosynthetic process; regulation of phagocytosis; recognition of apoptotic cell; lipopolysaccharide transport; cholesterol homeostasis; reverse cholesterol transport; detection of lipopolysaccharide; endothelial cell proliferation; lipopolysaccharide-mediated signaling pathway; adhesion to host; phospholipid transport; cholesterol catabolic process; cell adhesion

Disease: High Density Lipoprotein Cholesterol Level Quantitative Trait Locus 6