Western Blot (WB)
(HEK293 overexpressing SKAP2 (RC206119) and probed (mock transfection in first lane), tested by Origene.)
Goat SCAP2/PRAP Polyclonal Antibody | anti-SKAP2 antibody
Goat anti-SCAP2/PRAP Antibody
Western Blot: Approx 38kDa band observed in lysates of cell line Jurkat (calculated MW of 41.2kDa according to NP_003921.2). In transfected HEK293 transiently expressing SKAP2 a band of approx. 45kDa is observed. This band is not observed in the non-transfected HEK293.
Western Blot (WB)
(HEK293 overexpressing SKAP2 (RC206119) and probed (mock transfection in first lane), tested by Origene.)
Western Blot (WB)
(HEK293 overexpressing SKAP2 (RC206119) and probed (mock transfection in first lane), tested by Origene.)
Western Blot (WB)
((0.5ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.5ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene shares homology with Src kinase-associated phosphoprotein 1, and is a substrate of Src family kinases. It is an adaptor protein that is thought to play an essential role in the Src signaling pathway, and in regulating proper activation of the immune system. This protein contains an amino terminal coiled-coil domain for self-dimerization, a plecskstrin homology (PH) domain required for interactions with lipids at the membrane, and a Src homology (SH3) domain at the carboxy terminus. Some reports indicate that this protein inhibits actin polymerization through interactions with actin assembly factors, and might negatively regulate the invasiveness of tumors by modulating actin assembly. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2015]
Uniprot Description
SKAP2: an ubiquitous adapter protein thought to play a role in the src signaling pathway. Contains a coiled-coil domain, a pleckstrin homology domain, and a SH3 domain. The SH3 domain binds to the proline-rich domain of Pyk2, inhibiting alpha-synuclein tyrosine phosphorylation. Can be phosphorylated by Src family kinases but not by Pyk2. Functions as a downstream target for Pyk2 and plays a role in alpha-synuclein phosphorylation. Negatively regulates alpha-synuclein phosphorylation following cell stress.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 7p15.2
Cellular Component: cytoplasm; cytosol; nucleoplasm; plasma membrane
Molecular Function: protein binding; SH3/SH2 adaptor activity
Biological Process: B cell activation; immune response-activating signal transduction; negative regulation of cell proliferation; positive regulation of signal transduction; protein complex assembly; signal transduction
Research Articles on SKAP2
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Product Notes
The SKAP2 skap2 (Catalog #AAA421362) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-SCAP2/PRAP Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's SCAP2/PRAP can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 38kDa band observed in lysates of cell line Jurkat (calculated MW of 41.2kDa according to NP_003921.2). In transfected HEK293 transiently expressing SKAP2 a band of approx. 45kDa is observed. This band is not observed in the non-transfected HEK293. Researchers should empirically determine the suitability of the SKAP2 skap2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: GLVPKAYIME MYDI. It is sometimes possible for the material contained within the vial of "SCAP2/PRAP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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