Rabbit SCAP Polyclonal Antibody | anti-SCAP antibody

SCAP (Sterol Regulatory Element-binding Protein Cleavage-activating Protein, SREBP Cleavage-activating Protein, PSEC0227, KIAA0199) (MaxLight 490)

Cat. Num. AAA6498510

• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: SCAP; Polyclonal Antibody; SCAP (Sterol Regulatory Element-binding Protein Cleavage-activating Protein; SREBP Cleavage-activating Protein; PSEC0227; KIAA0199) (MaxLight 490); anti-SCAP antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SCAP. Species crossreactivity: mouse, rat
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-SCAP antibody: Immunohistochemistry (IHC) Paraffin, FLISA, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 604-632 in the central region of human SCAP, Swiss/UniProt Accession: Q12770. Species sequence homology: bovine, porcine
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of lysate from PC-3 cell line using MBS626672 (1:1000). IgG (HRP) goat anti-rabbit at 1:10,000 dilution was used as the secondary antibody. Lysate at 35ug.)

Western Blot (WB)

(Western Blot using MBS626672 (1:2000) Lane 1: Hela whole cell lysate Lane 2: HT-29 whole cell lysate Lane 3: K562 whole cell lysate Lane 4: LNCap whole cell lysate Lane 5: Mouse brain lysate Lane 6: Mouse liver lysate Lane 7: Rat heart lysate. Lysates/proteins at 20ug/lane. Secondary IgG (HRP) Goat Anti-Rabbit at 1:10,000. Predicted band size : 140, 98, 96kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(Immunohistochemistry of formalin-fixed, paraffin-embedded human colon carcinoma using MBS626672, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS626672 for immunohistochemistry.)

Immunofluorescence (IF)

(Fluorescent confocal image of HeLa cells using MBS626672. HeLa cells were fixed with 4% PFA (20min), permeabilized with Triton X-100 (0.2%, 30min). Cells were then incubated with MBS626672 (1:100, 2hr at RT). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1hr). Nuclei were counterstained with Hoechst 33342 (blue) (10ug/ml, 5min).)

Product Categories/Family for anti-SCAP antibody

Antibodies; Protein Kinases

References

1. Lu, Y., et.al., J. Lipid Res. 49(12), 2582-2589 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 66932902
• NCBI GeneID: 22937
• NCBI Accession #: NP_036367.2
• NCBI GenBank Nucleotide #: NM_012235.2
• UniProt Accession #: Q12770; Q8N2E0; Q8WUA1
• Molecular Weight: 95,616 Da
• NCBI Official Full Name: sterol regulatory element-binding protein cleavage-activating protein
• NCBI Official Synonym Full Names: SREBF chaperone
• NCBI Official Symbol: SCAP
• NCBI Protein Information: sterol regulatory element-binding protein cleavage-activating protein; SREBP cleavage-activating protein; sterol regulatory element binding protein cleavage-activating protein
• UniProt Protein Name: Sterol regulatory element-binding protein cleavage-activating protein
• Protein Family: Sterol regulatory element-binding protein cleavage-activating protein
• UniProt Gene Name: SCAP
• UniProt Synonym Gene Names: KIAA0199; SCAP; SREBP cleavage-activating protein
• UniProt Entry Name: SCAP_HUMAN

NCBI Description

This gene encodes a protein with a sterol sensing domain (SSD) and seven WD domains. In the presence of cholesterol, this protein binds to sterol regulatory element binding proteins (SREBPs) and mediates their transport from the ER to the Golgi. The SREBPs are then proteolytically cleaved and regulate sterol biosynthesis. [provided by RefSeq, Jul 2008]

Uniprot Description

SCAP: Escort protein required for cholesterol as well as lipid homeostasis. Regulates export of the SCAP/SREBF complex from the ER upon low cholesterol. Formation of a ternary complex with INSIG at high sterol concentrations leads to masking of an ER-export signal in SCAP and retention of the complex in the ER. Low sterol concentrations trigger release of INSIG, a conformational change in the SSC domain of SCAP, unmasking of the ER export signal, recruitment into COPII-coated vesicles, transport to the Golgi complex, proteolytic cleavage of SREBF in the Golgi, release of the transcription factor fragment of SREBF from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway. Belongs to the WD repeat SCAP family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: Golgi membrane; Golgi apparatus; endoplasmic reticulum membrane; protein complex; endoplasmic reticulum; integral to membrane

Molecular Function: protein binding; unfolded protein binding; cholesterol binding; protein complex binding

Biological Process: cholesterol metabolic process; regulation of fatty acid biosynthetic process; response to hypoxia; positive regulation of low-density lipoprotein receptor biosynthetic process; SREBP-mediated signaling pathway; negative regulation of cholesterol biosynthetic process; response to insulin stimulus; aging