Rabbit anti-Human SAV1 Polyclonal Antibody | anti-SAV1 antibody

SAV1 (Protein Salvador Homolog 1, SAV, 45 kDa WW Domain Protein, WW45, hWW45, WWP4)

Cat. Num. AAA646572

• Gene Names: SAV1; SAV; WW45; WWP4
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: SAV1; Polyclonal Antibody; SAV1 (Protein Salvador Homolog 1; SAV; 45 kDa WW Domain Protein; WW45; hWW45; WWP4); Anti -SAV1 (Protein Salvador Homolog 1; anti-SAV1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SAV1.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MLSRKKTKNEVSKPAEVQGKYVKKETSPLLRNLMPSFIRHGPTIPRRTDICLPDSSPNAFSTSGDVVSRNQSFLRTPIQRTPHEIMRRESNRLSAPSYLARSLADVPREYGSSQSFVTEVSFAVENGDSGSRYYYSDNFFDGQRKRPLGDRAHEDYRYYEYNHDLFQRMPQNQGRHASGIGRVAATSLGNLTNHGSEDLPLPPGWSVDWTMRGRKYYIDHNTNTTHWSHPLEREGLPPGWERVESSEFGTYYVDHTNKKAQYRHPCAPSVPRYDQPPPVTYQPQQTERNQSLLVPANPYHTAEIPDWLQVYARAPVKYDHILKWELFQLADLDTYQGMLKLLFMKELEQIVKMYEAYRQALLTELENRKQRQQWYAQQHGKNF

• Applicable Applications for anti-SAV1 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.
• Immunogen: Full length human SAV1, aa1-383 (NP_068590.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of SAV1 expression in transfected 293T cell line by SAV1 polyclonal antibody. Lane 1: SAV1 transfected lysate (44.6kD). Lane 2: Non-transfected lysate.)

Product Categories/Family for anti-SAV1 antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 48146711
• NCBI GeneID: 60485
• UniProt Accession #: Q9H4B6; Q6IA58; Q9H949; Q9HAK9; A8K4B8; D3DSB6
• Molecular Weight: 44,634 Da
• NCBI Official Full Name: SAV1
• NCBI Official Synonym Full Names: salvador homolog 1 (Drosophila)
• NCBI Official Symbol: SAV1
• NCBI Official Synonym Symbols: SAV; WW45; WWP4
• NCBI Protein Information: protein salvador homolog 1; hWW45; 1700040G09Rik; WW domain-containing; 45 kDa WW domain protein
• UniProt Protein Name: Protein salvador homolog 1
• Protein Family: Protein salvador
• UniProt Gene Name: SAV1
• UniProt Synonym Gene Names: WW45; hWW45
• UniProt Entry Name: SAV1_HUMAN

NCBI Description

WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein with two WW domains, a SARAH domain, and a coiled-coil region and is ubiquitously expressed in adult tissues. This protein binds to MST1 (mammalian sterile 20-like kinase 1) and promotes MST1-induced apoptosis. It has also been shown to bind to HAX1 (hematopoietic cell-specific protein 1 (HS1)-associated protein X-1) and to attenuate the anti-apoptotic effects of HAX1. Studies in human and mouse suggest this gene acts as a tumor suppressor. [provided by RefSeq, Aug 2012]

Uniprot Description

Function: Regulator of STK3/MST2 and STK4/MST1 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. SAV1 is required for STK3/MST2 and STK4/MST1 activation and promotes cell-cycle exit and terminal differentiation in developing epithelial tissues. Plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosomes, and its ability to phosphorylate CROCC and CEP250. In conjunction with STK3/MST2, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation. Ref.7 Ref.9 Ref.12 Ref.13

Subunit structure: Homodimer. Stabilized through interaction with STK3/MST2 or STK4/MST1. Interacts (via SARAH domain) with isoform 1 of NEK2. Interacts with ESR1 only in the presence of STK3/MST2. Interacts with WTIP and AJUBA. Ref.6 Ref.7 Ref.9 Ref.11 Ref.12 Ref.13

Subcellular location: Nucleus. Cytoplasm Ref.9.

Tissue specificity: Ubiquitously expressed in adult tissues with highest expression in the pancreas, aorta and interventricular septum and lowest expression in skeletal muscle. Expression was higher in fetal than in the adult heart. Expressed in various cell lines. Ref.9

Post-translational modification: Phosphorylated by STK3/MST2 and STK4/MST1. Phosphorylation is not required for SAV1 stability and may increase the number of protein binding sites on the scaffold molecule. Ref.7

Sequence similarities: Contains 1 SARAH domain.Contains 2 WW domains.