Chemiluminescence
((0.03ug/ml) staining of Hela lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat SART1 Polyclonal Antibody | anti-Sart1 antibody
SART1 (Squamous Cell Carcinoma Antigen Recognized by T Cells 1, SART-1, hSART-1, ARA1, Hom s 1, HOMS1, hSnu66, MGC2038, SART1-259, SART1259 Protein, SNRNP110, Snu66, U4/U6.U5 Tri-snRNP-associated 110kD Protein, U4/U6.U5 Tri-snRNP-associated Protein 1)
Gene Names
Sart1; U5-110K
Reactivity
Bovine, Human, Mouse, Rat
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
SART1; Polyclonal Antibody; SART1 (Squamous Cell Carcinoma Antigen Recognized by T Cells 1; SART-1; hSART-1; ARA1; Hom s 1; HOMS1; hSnu66; MGC2038; SART1-259; SART1259 Protein; SNRNP110; Snu66; U4/U6.U5 Tri-snRNP-associated 110kD Protein; U4/U6.U5 Tri-snRNP-associated Protein 1); Anti -SART1 (Squamous Cell Carcinoma Antigen Recognized by T Cells 1; anti-Sart1 antibody
Host
Goat
Reactivity
Bovine, Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Recognizes human SART1. Species sequence homology: Bovine, mouse and rat.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Applicable Applications for anti-Sart1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:64,000
Western Blot: 0.03-0.1ug/ml, observed in HeLa lysates on ~125kD bands
Dilution: ELISA: 1:64,000
Western Blot: 0.03-0.1ug/ml, observed in HeLa lysates on ~125kD bands
Immunogen
Synthetic peptide corresponding to GSSKKHRGEKEAA-C, from human SART1, at N-terminal (NP_005137.1).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Chemiluminescence
((0.03ug/ml) staining of Hela lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Chemiluminescence
((0.03ug/ml) staining of Hela lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Related Product Information for anti-Sart1 antibody
May play a role in mRNA splicing. May also bind to DNA.
Product Categories/Family for anti-Sart1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
90,885 Da
NCBI Official Full Name
squamous cell carcinoma antigen recognized by T cells
NCBI Official Synonym Full Names
squamous cell carcinoma antigen recognized by T cells 1
NCBI Official Symbol
Sart1
NCBI Official Synonym Symbols
U5-110K
NCBI Protein Information
U4/U6.U5 tri-snRNP-associated protein 1; SART-1; mSART-1; hypoxia-associated factor; squamous cell carcinoma antigen recognized by T-cells 1
UniProt Protein Name
U4/U6.U5 tri-snRNP-associated protein 1
Protein Family
UniProt Gene Name
Sart1
UniProt Synonym Gene Names
Haf; SART-1; mSART-1
UniProt Entry Name
SNUT1_MOUSE
Uniprot Description
SART1: May play a role in mRNA splicing. May also bind to DNA. Belongs to the SNU66/SART1 family. 1 isoforms of the human protein are produced by alternative splicing.
Protein type: RNA-binding; RNA processing; Spliceosome; RNA splicing; Apoptosis
Cellular Component: nucleoplasm; Golgi apparatus; spliceosome; Cajal body; U4/U6 x U5 tri-snRNP complex; nucleus
Biological Process: spliceosomal snRNP biogenesis; positive regulation of cytotoxic T cell differentiation; maturation of 5S rRNA; RNA splicing; nuclear mRNA cis splicing, via U2-type spliceosome; mRNA processing
Similar Products
Product Notes
The Sart1 sart1 (Catalog #AAA6008114) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The SART1 (Squamous Cell Carcinoma Antigen Recognized by T Cells 1, SART-1, hSART-1, ARA1, Hom s 1, HOMS1, hSnu66, MGC2038, SART1-259, SART1259 Protein, SNRNP110, Snu66, U4/U6.U5 Tri-snRNP-associated 110kD Protein, U4/U6.U5 Tri-snRNP-associated Protein 1) reacts with Bovine, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SART1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 1:64,000 Western Blot: 0.03-0.1ug/ml, observed in HeLa lysates on ~125kD bands. Researchers should empirically determine the suitability of the Sart1 sart1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SART1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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