Rabbit SARS Polyclonal Antibody | anti-SARS antibody

SARS, Spike Protein (CT) (Severe Acute Respiratory Syndrome, SARS-CoV, SARS Associated Coronavirus) (HRP)

Cat. Num. AAA6498475

• Gene Names: S; E2
• Applications: ELISA
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: SARS; Polyclonal Antibody; Spike Protein (CT) (Severe Acute Respiratory Syndrome; SARS-CoV; SARS Associated Coronavirus) (HRP); anti-SARS antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes the C-terminal of the spike protein of SARS-associated coronavirus.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-SARS antibody: ELISA (EIA)
• Application Notes: ELISA: 1ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to amino acids at the C-terminus of the SARS Spike glycoprotein.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Product Categories/Family for anti-SARS antibody

Antibodies; Infectious Disease SARS

References

1. Marra, MA., et al., (2003), "The Genome sequence of the SARS-associated corona virus", Science, 300, 1399-1404. 2. Rota, P.A., et al., (2003), "Characterization of a novel coronavirus associated with severe acute respiratory syndrome", Science, 300, 1394-1399. 3. Navas-Martin, S.R., et al., (2004), Coronavirus replication and pathogenesis: Implications for the recent outbreak of severe acute respiratory syndrome (SARS), and the challenge for vaccine development", J. Neurovirol., 10, 75-85. 4. Li, W., et al., (2003). "Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus", Nature, 426, 450-454.

NCBI and Uniprot Product Information

• NCBI GI #: 30173397
• NCBI GeneID: 1489668
• UniProt Accession #: P59594; Q6QU82; Q7T696; Q7TA19; Q7TFA2; Q7TFB1; Q80BV6
• Molecular Weight: 139,125 Da
• NCBI Official Full Name: Spike glycoprotein
• NCBI Official Symbol: S
• NCBI Official Synonym Symbols: E2
• NCBI Protein Information: E2 glycoprotein precursor; spike glycoprotein
• UniProt Protein Name: Spike glycoprotein
• Protein Family: Serine--tRNA ligase
• UniProt Gene Name: S
• UniProt Synonym Gene Names: S glycoprotein
• UniProt Entry Name: SPIKE_CVHSA

Uniprot Description

S1 attaches the virion to the cell membrane by interacting with human ACE2 and CLEC4M/DC-SIGNR, initiating the infection. Binding to the receptor and internalization of the virus into the endosomes of the host cell probably induces conformational changes in the S glycoprotein. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes.