Western Blot (WB)
((0. 03ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat SAR1B/SARA2 Polyclonal Antibody | anti-SAR1B antibody
Goat anti-SAR1B/SARA2 Antibody
Western Blot: Approx.25kDa band observed in Mouse Liver and Rat Liver lysates (calculated MW of 22.4kDa according to human NP_057187.1 and mouse NP_079811.1). In transfected HEK293 transiently expressing SAR1B a band of approx.26kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0. 03-0.1ug/ml.
Western Blot (WB)
((0. 03ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0. 03ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
(HEK293 overexpressing SAR1B (RC210593) and probed (mock transfection in first lane), tested by Origene.)
Western Blot (WB)
(HEK293 overexpressing SAR1B (RC210593) and probed (mock transfection in first lane), tested by Origene.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a small GTPase that acts as a homodimer. The encoded protein is activated by the guanine nucleotide exchange factor PREB and is involved in protein transport from the endoplasmic reticulum to the Golgi. This protein is part of the COPII coat complex. Defects in this gene are a cause of chylomicron retention disease (CMRD), also known as Anderson disease (ANDD). Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Mar 2010]
Uniprot Description
SAR1B: Involved in transport from the endoplasmic reticulum to the Golgi apparatus. Activated by the guanine nucleotide exchange factor PREB. Involved in the selection of the protein cargo and the assembly of the COPII coat complex. Defects in SAR1B are the cause of chylomicron retention disease (CMRD); also known as Anderson disease (ANDD). CMRD is an autosomal recessive disorder of severe fat malabsorption associated with failure to thrive in infancy. The condition is characterized by deficiency of fat-soluble vitamins, low blood cholesterol levels, and a selective absence of chylomicrons from blood. Affected individuals accumulate chylomicron-like particles in membrane-bound compartments of enterocytes, which contain large cytosolic lipid droplets. Belongs to the small GTPase superfamily. SAR1 family.
Protein type: Motility/polarity/chemotaxis; G protein, monomeric; G protein, monomeric, SAR1; G protein
Chromosomal Location of Human Ortholog: 5q31.1
Cellular Component: cytosol; endoplasmic reticulum membrane
Molecular Function: GTP binding; GTPase activity; metal ion binding
Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; antigen processing and presentation of peptide antigen via MHC class I; COPII coating of Golgi vesicle; ER to Golgi vesicle-mediated transport; intracellular protein transport; small GTPase mediated signal transduction
Disease: Chylomicron Retention Disease
Research Articles on SAR1B
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Product Notes
The SAR1B sar1b (Catalog #AAA420308) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-SAR1B/SARA2 Antibody reacts with Tested: Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, and may cross-react with other species as described in the data sheet. AAA Biotech's SAR1B/SARA2 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 8000. Western Blot: Approx.25kDa band observed in Mouse Liver and Rat Liver lysates (calculated MW of 22.4kDa according to human NP_057187.1 and mouse NP_079811.1). In transfected HEK293 transiently expressing SAR1B a band of approx.26kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0. 03-0.1ug/ml. Researchers should empirically determine the suitability of the SAR1B sar1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DHERLLESKE ELD. It is sometimes possible for the material contained within the vial of "SAR1B/SARA2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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