Western Blot (WB)
(Western blot analysis using MBS620807. HRP-labelled sheep anti-rabbit was used as the secondary antibody and the immunocomplex was then visualised by DAB. The expected molecular weight of the purified saporin is 29.5kD. SC: Spinal Cord homogenate (rat).)
Rabbit Saporin Polyclonal Antibody | anti-SAP2 antibody
Saporin (Saponaria officinalis, Common Soapwort)
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
SerumSerum
Synonyms
Saporin; Polyclonal Antibody; Saporin (Saponaria officinalis; Common Soapwort); Anti -Saporin (Saponaria officinalis; anti-SAP2 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes Saporin. Confirmed to react with purified Saporin.
Purity/Purification
Serum
Serum
Serum
Form/Format
Supplied as a lyophilized powder. Reconstitute in 100ul of sterile water. Centrifuge to remove any insoluble material.
Applicable Applications for anti-SAP2 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence.
Immunogen
Saporin, whole molecule.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis using MBS620807. HRP-labelled sheep anti-rabbit was used as the secondary antibody and the immunocomplex was then visualised by DAB. The expected molecular weight of the purified saporin is 29.5kD. SC: Spinal Cord homogenate (rat).)
Western Blot (WB)
(Western blot analysis using MBS620807. HRP-labelled sheep anti-rabbit was used as the secondary antibody and the immunocomplex was then visualised by DAB. The expected molecular weight of the purified saporin is 29.5kD. SC: Spinal Cord homogenate (rat).)
Related Product Information for anti-SAP2 antibody
Saporin is a ribosome-inactivating protein (RIP) of type I. This monomeric RNA N-glycosidase purified from seeds of the plant Saponaria officinalis also known as Soapwort, is capable of specific depurination of eukaryotic ribosomes thus arresting protein synthesis. No ligand has been identified in saporin hence its inability to transverse the cell membrane. Due to its toxicity and stability of the structure, saporin has proven extremely useful for construction of immunotoxins. The expected molecular weight of the purified saporin is 29.5kD.
Product Categories/Family for anti-SAP2 antibody
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
32,811 Da
NCBI Official Full Name
saporin
UniProt Protein Name
Ribosome-inactivating protein saporin-2
Protein Family
UniProt Gene Name
SAP2
UniProt Entry Name
RIP2_SAPOF
Uniprot Description
Function: Ribosome-inactivating protein of type 1, inhibits protein synthesis in animal cells. Useful as immunotoxin for pharmacological applications.
Catalytic activity: Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Sequence similarities: Belongs to the ribosome-inactivating protein family. Type 1 RIP subfamily.
Similar Products
Product Notes
The Saporin sap2 (Catalog #AAA620807) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Saporin can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence. Researchers should empirically determine the suitability of the Saporin sap2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Saporin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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