Rabbit anti-Human SAMSN1 Polyclonal Antibody | anti-SAMSN1 antibody

SAMSN1, ID (SAMSN1, HACS1, SAM domain-containing protein SAMSN-1, Hematopoietic adaptor containing SH3 and SAM domains 1, Nash1, SAM domain, SH3 domain and nuclear localization signals protein 1, SH3-SAM adaptor protein) (APC)

Cat. Num. AAA6344976

• Gene Names: SAMSN1; SLy2; HACS1; NASH1; SASH2; SH3D6B
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: SAMSN1; Polyclonal Antibody; ID (SAMSN1; HACS1; SAM domain-containing protein SAMSN-1; Hematopoietic adaptor containing SH3 and SAM domains 1; Nash1; SAM domain; SH3 domain and nuclear localization signals protein 1; SH3-SAM adaptor protein) (APC); SH3-SAM adaptor protein; anti-SAMSN1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SAMSN1.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-SAMSN1 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 255-283 in the Central region of human SAMSN1, UniProt Accession #Q9NSI8
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in Jurkat cell line lysates (35ug/lane) usingMBS6001563. SAMSN1 (arrow) was detected usingMBS6001563.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human esophagus carcinoma using MBS6001563 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6001563 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Jurkat cells using MBS6001563 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-SAMSN1 antibody

Antibodies; Nuclear Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 48762709
• NCBI GeneID: 64092
• NCBI Accession #: NP_071419.3
• NCBI GenBank Nucleotide #: NM_022136.4
• UniProt Accession #: Q9NSI8; Q8NFF7; Q9C041; B3KWJ3; F8WAA1
• Molecular Weight: 49,627 Da
• NCBI Official Full Name: SAM domain-containing protein SAMSN-1 isoform 1
• NCBI Official Synonym Full Names: SAM domain, SH3 domain and nuclear localization signals 1
• NCBI Official Symbol: SAMSN1
• NCBI Official Synonym Symbols: SLy2; HACS1; NASH1; SASH2; SH3D6B
• NCBI Protein Information: SAM domain-containing protein SAMSN-1
• UniProt Protein Name: SAM domain-containing protein SAMSN-1
• Protein Family: SAM domain-containing protein
• UniProt Gene Name: SAMSN1
• UniProt Synonym Gene Names: HACS1
• UniProt Entry Name: SAMN1_HUMAN

NCBI Description

SAMSN1 is a member of a novel gene family of putative adaptors and scaffold proteins containing SH3 and SAM (sterile alpha motif) domains (Claudio et al., 2001 [PubMed 11536050]).[supplied by OMIM, Mar 2008]

Uniprot Description

SAMSN1: an adaptor and scaffold protein containing an SH3 and a SAM (sterile alpha motif) domain. Its expression is induced by B cell activation signals. Two alternatively spliced isoforms have been described.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 21q11

Cellular Component: ruffle; cytosol; nucleus

Molecular Function: phosphotyrosine binding

Biological Process: negative regulation of peptidyl-tyrosine phosphorylation; negative regulation of adaptive immune response; negative regulation of B cell activation