Western Blot (WB)
(Western blot analysis of extracts from various samples, using SAMHD1 Antibody.Lane 1: Hela cells(heat shock treatment), blocked with antigen-specific peptides,Lane 2: Hela cells(heat shock treatment),Lane 3: HepG2 cells(serum starvation treatment).)
Rabbit SAMHD1 Polyclonal Antibody | anti-SAMHD1 antibody
SAMHD1 Antibody
Gene Names
SAMHD1; DCIP; CHBL2; HDDC1; MOP-5; SBBI88
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Synonyms
SAMHD1; Polyclonal Antibody; SAMHD1 Antibody; CHBL2; DCIP; Dendritic cell derived IFNG induced protein; Dendritic cell-derived IFNG-induced protein; Deoxynucleoside triphosphate triphosphohydrolase SAMHD1; dNTPase; HD domain containing 1; HDDC1; Mg11; Monocyte protein 5; MOP 5; MOP-5; MOP5; OTTHUMP00000030889; SAM domain and HD domain 1; SAM domain and HD domain containing protein 1; SAM domain and HD domain-containing protein 1; SAMH1_HUMAN; Samhd1; SBBI88; anti-SAMHD1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
SAMHD1 Antibody detects endogenous levels of total SAMHD1.
Tissue Specificity: Expressed in heart, skeletal muscle, spleen, liver, small intestine, placenta, lung and peripheral blood leukocytes. No expression is seen in brain and thymus.
Tissue Specificity: Expressed in heart, skeletal muscle, spleen, liver, small intestine, placenta, lung and peripheral blood leukocytes. No expression is seen in brain and thymus.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-SAMHD1 antibody
Western Blot (WB), Peptide ELISA (EIA)
Application Notes
WB: 1:500-1:2000
Peptide ELISA: 1:20,000-1:40,000
Peptide ELISA: 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human SAMHD1.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
Phosphorylation at Thr-592 by CDK1 acts as a switch to control deoxynucleoside triphosphate (dNTPase)-dependent and -independent functions. Phosphorylation at Thr-592 takes place in cycling cells: it reduces the stability of the homotetramer, impairing the dNTPase activity and subsequent ability to restrict infection by viruses. It also inhibits ability to suppress LINE-1 retrotransposon activity. In contrast, phosphorylation at Thr-592 promotes DNA end resection at stalled replication forks in response to DNA damage. (Microbial infection) Ubiquitinated following interaction with HIV-2 viral protein Vpx; Vpx promotes interaction and with a DCX (DDB1-CUL4-X-box) E3 ubiquitin ligase, leading to proteasomal degradation.
Subunit Structure
Homodimer; in absence of GTP and dNTP. Homotetramer; in GTP- and dNTP-bound form. Interacts with MRE11; leading to stimulate the exonuclease activity of MRE11. Interacts with RBBP8/CtIP. Interacts (via its C-terminus) with CD81. (Microbial infection) Interacts with HIV-2 viral protein Vpx; promoting interaction with a E3 ubiquitin-protein ligase complex containing DCAF1, leading to subsequent ubiquitination and degradation of SAMHD1.
Similarity
In human, and in contrast to mouse protein, the SAM domain is not required for deoxynucleoside triphosphate (dNTPase) activity and ability to restrict infection by viruses.Belongs to the SAMHD1 family.
Subcellular Location
Nucleus. Chromosome.
Note: Localizes to sites of DNA double-strand breaks in response to DNA damage.
Note: Localizes to sites of DNA double-strand breaks in response to DNA damage.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from various samples, using SAMHD1 Antibody.Lane 1: Hela cells(heat shock treatment), blocked with antigen-specific peptides,Lane 2: Hela cells(heat shock treatment),Lane 3: HepG2 cells(serum starvation treatment).)
Western Blot (WB)
(Western blot analysis of extracts from various samples, using SAMHD1 Antibody.Lane 1: Hela cells(heat shock treatment), blocked with antigen-specific peptides,Lane 2: Hela cells(heat shock treatment),Lane 3: HepG2 cells(serum starvation treatment).)
Western Blot (WB)
(Western blot analysis of extracts from heat-shock treated 293 cells, using SAMHD1 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from heat-shock treated 293 cells, using SAMHD1 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-SAMHD1 antibody
Protein that acts both as a host restriction factor involved in defense response to virus and as a regulator of DNA end resection at stalled replication forks. Has deoxynucleoside triphosphate (dNTPase) activity, which is required to restrict infection by viruses, such as HIV-1: dNTPase activity reduces cellular dNTP levels to levels too low for retroviral reverse transcription to occur, blocking early-stage virus replication in dendritic and other myeloid cells. Likewise, suppresses LINE-1 retrotransposon activity. Not able to restrict infection by HIV-2 virus; because restriction activity is counteracted by HIV-2 viral protein Vpx. In addition to virus restriction, dNTPase activity acts as a regulator of DNA precursor pools by regulating dNTP pools. Phosphorylation at Thr-592 acts as a switch to control dNTPase-dependent and -independent functions: it inhibits dNTPase activity and ability to restrict infection by viruses, while it promotes DNA end resection at stalled replication forks. Functions during S phase at stalled DNA replication forks to promote the resection of gapped or reversed forks: acts by stimulating the exonuclease activity of MRE11, activating the ATR-CHK1 pathway and allowing the forks to restart replication. Its ability to promote degradation of nascent DNA at stalled replication forks is required to prevent induction of type I interferons, thereby preventing chronic inflammation. Ability to promote DNA end resection at stalled replication forks is independent of dNTPase activity. Enhances immunoglobulin hypermutation in B-lymphocytes by promoting transversion mutation (By similarity).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 65; 69 kDa
Observed: 62 kDa
Observed: 62 kDa
NCBI Official Full Name
deoxynucleoside triphosphate triphosphohydrolase SAMHD1
NCBI Official Synonym Full Names
SAM domain and HD domain 1
NCBI Official Symbol
SAMHD1
NCBI Official Synonym Symbols
DCIP; CHBL2; HDDC1; MOP-5; SBBI88
NCBI Protein Information
deoxynucleoside triphosphate triphosphohydrolase SAMHD1; dNTPase; monocyte protein 5; dendritic cell-derived IFNG-induced protein; SAM domain and HD domain-containing protein 1
UniProt Protein Name
Deoxynucleoside triphosphate triphosphohydrolase SAMHD1
Protein Family
UniProt Gene Name
SAMHD1
UniProt Synonym Gene Names
MOP5; dNTPase; DCIP; MOP-5
UniProt Entry Name
SAMH1_HUMAN
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Product Notes
The SAMHD1 samhd1 (Catalog #AAA9613530) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SAMHD1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SAMHD1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Peptide ELISA (EIA). WB: 1:500-1:2000 Peptide ELISA: 1:20,000-1:40,000. Researchers should empirically determine the suitability of the SAMHD1 samhd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SAMHD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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