Rabbit anti-Mouse SAMD8 Polyclonal Antibody | anti-SAMD8 antibody

SAMD8, NT (SAMD8, Sphingomyelin synthase-related protein 1, Sterile alpha motif domain-containing protein 8) (PE)

Cat. Num. AAA6344930

• Gene Names: SAMD8; SMSr; HEL-177
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SAMD8; Polyclonal Antibody; NT (SAMD8; Sphingomyelin synthase-related protein 1; Sterile alpha motif domain-containing protein 8) (PE); Sterile alpha motif domain-containing protein 8; anti-SAMD8 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-SAMD8 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SAMD8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 45-75 amino acids from the N-terminal region of human SAMD8.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of SAMD8 Antibody (N-term) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in mouse liver tissue lysate. SAMD8 Antibody (N-term) (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-SAMD8 antibody

Antibodies; Enzymes; Synthase

References

Grupe, A., et al. Am. J. Hum. Genet. 78(1):78-88(2006). Barrios-Rodiles, M., et al. Science 307(5715):1621-1625(2005). Huitema, K., et al. EMBO J. 23(1):33-44(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 21389545
• NCBI GeneID: 142891
• NCBI Accession #: NP_653261.1
• NCBI GenBank Nucleotide #: NM_144660.2
• UniProt Accession #: Q96LT4; Q5JSC5; Q5JSC8; Q66K52
• Molecular Weight: 37,609 Da
• NCBI Official Full Name: sphingomyelin synthase-related protein 1 isoform 2
• NCBI Official Synonym Full Names: sterile alpha motif domain containing 8
• NCBI Official Symbol: SAMD8
• NCBI Official Synonym Symbols: SMSr; HEL-177
• NCBI Protein Information: sphingomyelin synthase-related protein 1
• UniProt Protein Name: Sphingomyelin synthase-related protein 1
• Protein Family: Sphingomyelin synthase-related protein
• UniProt Gene Name: SAMD8
• UniProt Synonym Gene Names: SMSr; CPE synthase
• UniProt Entry Name: SAMD8_HUMAN

Uniprot Description

SAMD8: Sphingomyelin synthases synthesize sphingolipids through transfer of a phosphatidyl head group on to the primary hydroxyl of ceramide. SAMD8 is an endoplasmic reticulum (ER) transferase that has no sphingomyelin synthase activity but can convert phosphatidylethanolamine (PE) and ceramide to ceramide phosphoethanolamine (CPE) albeit with low product yield. Appears to operate as a ceramide sensor to control ceramide homeostasis in the endoplasmic reticulum rather than a converter of ceramides. Seems to be critical for the integrity of the early secretory pathway. Belongs to the sphingomyelin synthase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 10q22.2

Cellular Component: integral to membrane; integral to endoplasmic reticulum membrane

Molecular Function: transferase activity

Biological Process: ceramide biosynthetic process; sphingomyelin biosynthetic process