Rabbit SALL1 Polyclonal Antibody | anti-SALL1 antibody

SALL1 Antibody

Cat. Num. AAA9612217

• Gene Names: SALL1; TBS; HSAL1; Sal-1; ZNF794; HEL-S-89
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: SALL1; Polyclonal Antibody; SALL1 Antibody; HSal1; Sal like protein 1; Sal-1; Sal-like protein 1; Sal1; SALL1_HUMAN; Spalt-like transcription factor 1; TBS; Zinc finger protein 794; Zinc finger protein SALL1; Zinc finger protein Spalt-1; anti-SALL1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SALL1 Antibody detects endogenous levels of total SALL1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 1324

• Applicable Applications for anti-SALL1 antibody: ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IF: 1:100-1:500; ICC: 1:100-1:500; ELISA(peptide): 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human SALL1, corresponding to a region within N-terminal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Myeloma cells, using SALL1 Antibody. The lane on the left was treated with blocking peptide.)

Immunofluorescence (IF)/Immunocytochemistry (ICC)

(MBS9612217 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9612217) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

Related Product Information for anti-SALL1 antibody

Function: Transcriptional repressor involved in organogenesis.

Subcellular Location: Nucleus.

Tissue Specificity: Highest levels in kidney. Lower levels in adult brain (enriched in corpus callosum, lower expression in substantia nigra) and liver.

Subunit Structure: Interacts with HDAC1, HDAC2, RBBP4, RBPP7, MTA1 and MTA2 (By similarity). Interacts with CCNQ. Probably associates with NuRD histone deacetylase complex (HDAC).

Similarity: Belongs to the sal C2H2-type zinc-finger protein family.

NCBI and Uniprot Product Information

• NCBI GI #: 118442837
• NCBI GeneID: 6299
• NCBI Accession #: NP_002959.2
• NCBI GenBank Nucleotide #: NP_002959.2
• UniProt Accession #: Q9NSC2
• Molecular Weight: Observed Molecular Weight: (Observed)140kD.; Predicted Molecular Weight: (Calculated)140kDa.
• NCBI Official Full Name: sal-like protein 1 isoform a
• NCBI Official Synonym Full Names: spalt like transcription factor 1
• NCBI Official Symbol: SALL1
• NCBI Official Synonym Symbols: TBS; HSAL1; Sal-1; ZNF794; HEL-S-89
• NCBI Protein Information: sal-like protein 1
• UniProt Protein Name: Sal-like protein 1
• Protein Family: Sal-like protein
• UniProt Gene Name: SALL1
• UniProt Synonym Gene Names: SAL1; ZNF794
• UniProt Entry Name: SALL1_HUMAN

NCBI Description

The protein encoded by this gene is a zinc finger transcriptional repressor and may be part of the NuRD histone deacetylase complex (HDAC). Defects in this gene are a cause of Townes-Brocks syndrome (TBS) as well as bronchio-oto-renal syndrome (BOR). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]