Western Blot (WB)
(Western Blot analysis of extracts of various cell lines, using SAFB2 antibody.)
Rabbit SAFB2 Polyclonal Antibody | anti-SAFB2 antibody
SAFB2 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
SAFB2; Polyclonal Antibody; SAFB2 Polyclonal Antibody; Scaffold attachment factor B2; SAF-B2; KIAA0138; anti-SAFB2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
SAFB2 polyclonal antibody detects endogenous levels of SAFB2 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
953
Applicable Applications for anti-SAFB2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
Recombinant protein of human SAFB2.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot analysis of extracts of various cell lines, using SAFB2 antibody.)
Western Blot (WB)
(Western Blot analysis of extracts of various cell lines, using SAFB2 antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human prostate using SAFB2 antibody at dilution of 1:100 (x40 lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human prostate using SAFB2 antibody at dilution of 1:100 (x40 lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded rat brain using SAFB2 antibody at dilution of 1:100 (x40 lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded rat brain using SAFB2 antibody at dilution of 1:100 (x40 lens).)
Related Product Information for anti-SAFB2 antibody
SAF-B2 (scaffold attachment factor B2) is a nuclear matrix-associated protein that binds to matrix- or scaffold-associating regions (MARs or SARs) on DNA and interacts with RNA polymerase II and serine-/arginine-rich RNA processing factors (SR proteins). SAF-B2, and the related SAF-B, bind to the estrogen receptor (ER) and are expressed in several breast cancer cell lines at varying levels. Subsequently, SAF-B and SAF-B2 may play a role in breast cancer by mediating cellular proliferation and division. Unlike SAF-B, which is exclusively nuclear, SAF-B2 is found in the cytoplasm as well as the nucleus.
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
~ 160kDa
NCBI Official Full Name
Scaffold attachment factor B2
UniProt Protein Name
Scaffold attachment factor B2
Protein Family
UniProt Gene Name
SAFB2
UniProt Synonym Gene Names
KIAA0138; SAF-B2
UniProt Entry Name
SAFB2_HUMAN
Uniprot Description
SAFB2: Binds to scaffold/matrix attachment region (S/MAR) DNA. Can function as an estrogen receptor corepressor and can also inhibit cell proliferation.
Protein type: Nuclear receptor co-regulator; RNA-binding
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: nucleoplasm; intracellular membrane-bound organelle; cytoplasm
Molecular Function: identical protein binding; protein binding; DNA binding; nucleotide binding
Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent
Similar Products
Product Notes
The SAFB2 safb2 (Catalog #AAA3008592) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SAFB2 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SAFB2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the SAFB2 safb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SAFB2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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