Western Blot (WB)
(Western blot analysis of the extracts from 293 cells untreated or treated with serum (10%, 15min) using S6 antibody (Ser235) (Lane 1 and 2) and S6 (phospho-Ser235) antibody (Lane 3 and 4).)
Rabbit S6 Polyclonal Antibody | anti-S6 antibody
S6 antibody (Ser235)
Applications
Western Blot, ELISA
Synonyms
S6; Polyclonal Antibody; S6 antibody (Ser235); Polyclonal S6; Anti-S6; 40S ribosomal protein S6; Phosphoprotein NP33; RPS6; RS6; ribosomal protein S6; anti-S6 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
S6 antibody (Ser235) detects endogenous levels of S6 Ribosomal protein only when phosphorylated at serine 235
Form/Format
Purified IgG supplied in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-S6 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
ELISA: 1:20000
ELISA: 1:20000
Biological Significance
S6 may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.
Cross-Reactivity
Mouse, Rat
Immunogen
S6 antibody was raised in Rabbit using synthesized phosphopeptide derived from human S6 Ribosomal protein around the phosphorylation site of serine 235 (R-L-SP-S-L) as the immunogen
Preparation and Storage
Store in small aliquots at -20 degree C. Avoid repeated freeze/thaw cycles
Western Blot (WB)
(Western blot analysis of the extracts from 293 cells untreated or treated with serum (10%, 15min) using S6 antibody (Ser235) (Lane 1 and 2) and S6 (phospho-Ser235) antibody (Lane 3 and 4).)
Western Blot (WB)
(Western blot analysis of the extracts from 293 cells untreated or treated with serum (10%, 15min) using S6 antibody (Ser235) (Lane 1 and 2) and S6 (phospho-Ser235) antibody (Lane 3 and 4).)
Related Product Information for anti-S6 antibody
Rabbit polyclonal S6 antibody (Ser235)
Product Categories/Family for anti-S6 antibody
Similar Products
Product Notes
The S6 (Catalog #AAA5302867) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's S6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:1000 ELISA: 1:20000. Researchers should empirically determine the suitability of the S6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "S6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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