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Western Blot (WB) (Western blot analysis of S1PR2 in HeLa cell lysate with S1PR2 antibody at 1 μg/ml.)

Rabbit S1PR2 Polyclonal Antibody | anti-S1PR2 antibody

S1PR2 Antibody

Gene Names
S1PR2; EDG5; H218; LPB2; S1P2; AGR16; EDG-5; DFNB68; Gpcr13
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity chromatography purified via peptide column.
Synonyms
S1PR2; Polyclonal Antibody; S1PR2 Antibody; Sphingosine 1-phosphate receptor 2; S1P2; AGR16; EDG-5; EDG5; Gpcr13; H218; LPB2; anti-S1PR2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
S1PR2 antibody is human, mouse and rat reactive.
Purity/Purification
Affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
353
Applicable Applications for anti-S1PR2 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
S1PR2 antibody can be used for detection of S1PR2 by Western blot at 1 - 2 mug/ml. Antibody can also be used for immunohistochemistry at 10 mug/ml.
Immunogen
S1PR2 antibody was raised against an 16 amino acid peptide near the carboxy terminus of human S1PR2.
Buffer
Supplied in PBS containing 0.02% sodium azide.
Positive Control 1
HeLa Cell Lysate
Conjugate
Unconjugated
MW Explanation
Multiple transmembrane protein, also multiple other S1PR2 antibodies also show a ~50kD band
Preparation and Storage
Store at 4 degree C for three months and -20 degree C, stable for up to one year.

Western Blot (WB)

(Western blot analysis of S1PR2 in HeLa cell lysate with S1PR2 antibody at 1 μg/ml.)

Western Blot (WB) (Western blot analysis of S1PR2 in HeLa cell lysate with S1PR2 antibody at 1 μg/ml.)

Immunohistochemistry (IHC)

(Immunohistochemistry of S1PR2 in mouse brain tissue with S1PR2 antibody at 10 μg/ml.)

Immunohistochemistry (IHC) (Immunohistochemistry of S1PR2 in mouse brain tissue with S1PR2 antibody at 10 μg/ml.)

Immunofluorescence (IF)

(Immunofluorescence of S1PR2 in mouse brain tissue with S1PR2 antibody at 20 μg/ml.Red: S1PR2 Antibody (8229)Blue: DAPI staining)

Immunofluorescence (IF) (Immunofluorescence of S1PR2 in mouse brain tissue with S1PR2 antibody at 20 μg/ml.Red: S1PR2 Antibody (8229)Blue: DAPI staining)
Related Product Information for anti-S1PR2 antibody
The Sphingosine 1-phosphate receptor 2 (S1PR2) protein was initially identified as G-protein coupled receptor thought to be involved in development (1) and was found to mediate sphingosine 1-phosphate (SPP)-induced cell rounding and neurite retraction (2). S1PR2 and the related protein S1PR3 (also known as EDG3) are also thought to mediate the SPP-induced cell proliferation, survival, and related signaling events (3). S1PR2 has also been found to be key regulator in acute vascular inflammation and may be a novel therapeutic target for vascular disorders (4).
Product Categories/Family for anti-S1PR2 antibody
References
MacLennan AJ, Browe CS, Gaskin AA, et al. Cloning and characterization of a putative G-protein coupled receptor potentially involved in development. Mol. Cell Neurosci. 1994; 5:201-9. Van Brocklyn JR, Tu Z, Edsall LC, et al. Sphingosine 1-phosphate-induced cell rounding and neurite retraction are mediated by the G protein-coupled receptor H218. J. Biol. Chem. 1999; 274:4626-32. An S, Zheng Y, and Bleu T. Sphingosine 1-phosphate-induced cell proliferation, survival, and related signaling events mediated by G protein-coupled receptors Edg3 and Edg5. J. Biol. Chem. 275:288-96. Zhang G, Yang L, Kim GS, et al. Critical role of sphingosine-1-phosphate receptor 2 (S1PR2) in acute vascular inflammation. Blood 2013; 122:443-55.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted: 39 kDa
Observed: 50 kDa
NCBI Official Full Name
sphingosine 1-phosphate receptor 2
NCBI Official Synonym Full Names
sphingosine-1-phosphate receptor 2
NCBI Official Symbol
S1PR2
NCBI Official Synonym Symbols
EDG5; H218; LPB2; S1P2; AGR16; EDG-5; DFNB68; Gpcr13
NCBI Protein Information
sphingosine 1-phosphate receptor 2
UniProt Protein Name
Sphingosine 1-phosphate receptor 2
UniProt Gene Name
S1PR2
UniProt Synonym Gene Names
EDG5; S1P receptor 2; S1P2; S1P receptor Edg-5
UniProt Entry Name
S1PR2_HUMAN

NCBI Description

This gene encodes a member of the G protein-coupled receptors, as well as the EDG family of proteins. The encoded protein is a receptor for sphingosine 1-phosphate, which participates in cell proliferation, survival, and transcriptional activation. Defects in this gene have been associated with congenital profound deafness. [provided by RefSeq, Mar 2016]

Uniprot Description

EDG5: Receptor for the lysosphingolipid sphingosine 1- phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. When expressed in rat HTC4 hepatoma cells, is capable of mediating S1P-induced cell proliferation and suppression of apoptosis. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, GPCR; Membrane protein, multi-pass; Lipid-binding

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: integral to membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; G-protein-coupled receptor binding; integrin binding; lipid binding; protein binding

Biological Process: actin cytoskeleton reorganization; activation of MAPK activity; filopodium formation; G-protein coupled receptor protein signaling pathway; positive regulation of cell proliferation

Research Articles on S1PR2

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Product Notes

The S1PR2 s1pr2 (Catalog #AAA154380) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The S1PR2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's S1PR2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). S1PR2 antibody can be used for detection of S1PR2 by Western blot at 1 - 2 mug/ml. Antibody can also be used for immunohistochemistry at 10 mug/ml. Researchers should empirically determine the suitability of the S1PR2 s1pr2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "S1PR2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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