Western Blot (WB)
((0. 05ug/ml) staining of Peripheral blood mononuclear cells lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat S100A4/CAPL Polyclonal Antibody | anti-S100A4 antibody
Goat anti-S100A4/CAPL Antibody
Immunohistochemistry: In paraffin embedded Human Small Intestine shows staining of lymphatic cells in the lamina propria. Recommended concentration, 1-2ug/ml.
Western Blot: Approx 13kDa band observed in Human Peripheral blood mononuclear cells lysates (calculated MW of 11.7kDa according to NP_002952.1). Recommended concentration: 0. 05-0.2ug/ml.
Western Blot (WB)
((0. 05ug/ml) staining of Peripheral blood mononuclear cells lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0. 05ug/ml) staining of Peripheral blood mononuclear cells lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
((2ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)
Immunohistochemistry (IHC)
((2ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]
Uniprot Description
S100A4: is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]
Protein type: Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 1q21
Cellular Component: extracellular space; neuron projection; nucleus; perinuclear region of cytoplasm
Molecular Function: actin binding; calcium ion binding; calcium-dependent protein binding; identical protein binding; protein binding; RAGE receptor binding
Biological Process: epithelial to mesenchymal transition; positive regulation of I-kappaB kinase/NF-kappaB cascade
Research Articles on S100A4
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Product Notes
The S100A4 s100a4 (Catalog #AAA420725) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-S100A4/CAPL Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's S100A4/CAPL can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 128000. Immunohistochemistry: In paraffin embedded Human Small Intestine shows staining of lymphatic cells in the lamina propria. Recommended concentration, 1-2ug/ml. Western Blot: Approx 13kDa band observed in Human Peripheral blood mononuclear cells lysates (calculated MW of 11.7kDa according to NP_002952.1). Recommended concentration: 0. 05-0.2ug/ml. Researchers should empirically determine the suitability of the S100A4 s100a4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: CNEFFEGFPD KQP. It is sometimes possible for the material contained within the vial of "S100A4/CAPL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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