Sheep S100A1 (Calcium Binding Protein) Polyclonal Antibody | anti-S100A1 antibody

S100A1 Polyclonal Antibody

Cat. Num. AAA194349

• Gene Names: S100A1; S100; S100A; S100-alpha
• Applications: Western Blot, immunoassay, Radioimmunoassay, Immunohistochemistry, Immunocytochemistry
• Synonyms: S100A1 (Calcium Binding Protein); Polyclonal Antibody; S100A1 Polyclonal Antibody; Anti-S100A1 (Calcium Binding Protein); S100A1; anti-S100A1 antibody

• Host: Sheep
• Clonality: Polyclonal
• Specificity: Anti-S100A1 (Calcium Binding Protein) [Polyclonal (sheep) antibody recognizes bovine and porcine S100A1.]; This antibody reacts with bovine and porcine S100A1 in immunoblots. Immunoreactivity with recombinant rat and human S100A1 and recombinant human S100A4 is reduced when compared to native bovine and porcine S100A1. Does not react with human S100A2 in immunoblots. This antibody reacts with bovine, rat, and recombinant S100B in immunoblots but less than 5% crossreactivity with S100B is seen in competitition radioimmunoassays. Crossreactivity with other members of the S100 family has not been investigated.
• Form/Format: 200 ul IgG prepared by ammonium sulfate precipitation in PBS, pH 7.4.
• Sequence Length: 94

• Applicable Applications for anti-S100A1 antibody: Western Blot, Radioimmunoassay, Immunohistochemistry, Immunocytochemistry
• Application Notes: This antibody may be used in immunohistochemistry, immunocytochemistry, radioimmunoassays, and Western Blots to detect or quantitate S100A1
• Antigen: Alum denatured porcine S100A1.
• Stabilizer: None
• Preservatives: None. Available on request.
• Dilutions Instructions: Dilute in PBS or medium which is identical to that used in the assay system. For Western blots using an avidin-biotin secondary antibody system, a 1:50 dilution has been used successfully. For indirect immunofluorescence, a 1:2 dilution has been used successfully. Optimal dilutions should be determined for each assay system.
• Preparation and Storage: These antibodies are stable for at least one (1) year at -20 degree C to -70 degree C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles

Product Categories/Family for anti-S100A1 antibody

Neuroscience and Signal Transduction Antibodies

References

Zimmer and Landar. 1995. Jour Neurochemistry 64: 2727; Van Eldik et al. 1988. Jour Biol Chem 263: 7830.

NCBI and Uniprot Product Information

• NCBI GI #: 5454032
• NCBI GeneID: 6271
• NCBI Accession #: NP_006262.1
• NCBI GenBank Nucleotide #: NM_006271.1
• UniProt Accession #: P23297; Q5T7Y3; Q5T7Y4; Q5T7Y5; Q5T7Y6
• Molecular Weight: 10,546 Da
• NCBI Official Full Name: protein S100-A1
• NCBI Official Synonym Full Names: S100 calcium binding protein A1
• NCBI Official Symbol: S100A1
• NCBI Official Synonym Symbols: S100; S100A; S100-alpha
• NCBI Protein Information: protein S100-A1; S100 alpha; OTTHUMP00000035100; S-100 protein alpha chain; S-100 protein subunit alpha; S100 calcium-binding protein A1; S100 protein, alpha polypeptide
• UniProt Protein Name: Protein S100-A1
• Protein Family: Protein
• UniProt Gene Name: S100A1
• UniProt Synonym Gene Names: S100A
• UniProt Entry Name: S10A1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of protein kinase C-mediated phosphorylation. Reduced expression of this protein has been implicated in cardiomyopathies. [provided by RefSeq]

Uniprot Description

S100A1: Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain. Interacts with AGER and CAPZA1. Interacts with FKBP4. Interacts with RYR1 and RYR2. Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain. Belongs to the S-100 family.

Protein type: Calcium-binding; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: neuron projection; protein complex; sarcoplasmic reticulum; M band; nucleus; Z disc

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; calcium ion binding; calcium-dependent protein binding; ATPase binding

Biological Process: substantia nigra development; negative regulation of transcription from RNA polymerase II promoter; regulation of heart contraction