Rabbit anti-Human, Mouse RYBP Polyclonal Antibody | anti-RYBP antibody

RYBP Antibody

Cat. Num. AAA9630836

• Gene Names: RYBP; AAP1; DEDAF; YEAF1
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: RYBP; Polyclonal Antibody; RYBP Antibody; 2410018J24Rik; AAP 1; AAP1; APAP 1; APAP-1; APAP1; Apoptin associating protein 1; Apoptin-associating protein 1; cb337; Death effector domain associated factor; Death effector domain-associated factor; Death effector domain-associated factor B; DED associated factor; DED-associated factor; DED-associated factor B; DEDAF; MGC62213; RING1 and YY1 binding protein a; RING1 and YY1 binding protein; RING1 and YY1-binding protein; RING1 and YY1-binding protein B; Ring1 interactor RYBP; RYBP_HUMAN; rybpa; wu:fi34f03; YEAF 1; YEAF1; YY1 and E4TF1 associated factor 1; YY1 and E4TF1-associated factor 1; YY1- and E4TF1/GABP-associated factor 1; zgc:153842; anti-RYBP antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: RYBP Antibody detects endogenous levels of RYBP.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-RYBP antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human RYBP.
• Conjugation: Unconjugated
• Similarity: Intrinsically unstructured in the absence of binding partners. Folds upon binding to DNA or RNF2 (By similarity).
• Post Translational Modifications: Monoubiquitinated.
• Subunit Structure: Monomer. Component of repressive BCOR complex containing Polycomb group subcomplex at least composed of BCOR, PCGF1, RING1 and RNF2/RING2. Component of PCR1-like complexes. Interacts with PCGF1. Part of a PCR1-like complex that contains AUTS2, PCGF5, RNF2, CSNK2B AND RYBP. Interacts with RNF2; the interaction is direct. Interacts with CBX2, YAF2, RING1 and RNF2 (By similarity). Interacts with ubiquitin and ubiquitinated proteins (By similarity). Interacts with ubiquitinated histone H2A (By similarity). Interacts with apoptin, DEDD, FADD, CASP8, CASP10, YY1 and GABPB1. Together with GABPB1 and YY1, it forms a ternary complex, probably being the bridge factor between these two transcription factors. Interacts with MDM2, and thereby inhibits ubiquitination of TP53. Identified in a ternary complex containing MDM2, TP53 and RYBP. Interacts with FANK1; may prevent the ubiquitin-mediated proteasomal degradation of FANK1. Interacts with IFT57 (By similarity).
• Tissue Specificity: Down-regulated in breast cancer tissues and in several breast cancer cell lines (at protein level). Widely expressed with highest levels in lymphoid tissues and placenta.
• Function: Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1-like complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Component of a PRC1-like complex that mediates monoubiquitination of histone H2A 'Lys-119' on the X chromosome and is required for normal silencing of one copy of the X chromosome in XX females. May stimulate ubiquitination of histone H2A 'Lys-119' by recruiting the complex to target sites (By similarity). Inhibits ubiquitination and subsequent degradation of TP53, and thereby plays a role in regulating transcription of TP53 target genes. May also regulate the ubiquitin-mediated proteasomal degradation of other proteins like FANK1 to regulate apoptosis. May be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1. May bind to DNA (By similarity). May play a role in the repression of tumor growth and metastasis in breast cancer by down-regulating SRRM3.
• Note: Primarily found in the nucleus. Detected in a punctate pattern likely to represent Polycomb group (PcG) bodies (By similarity).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-RYBP antibody

Nucleus; Cytoplasm; Nucleus; Nucleoplasm; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 112363092
• NCBI GeneID: 23429
• NCBI Accession #: NP_036366.3
• NCBI GenBank Nucleotide #: NM_012234.6
• UniProt Accession #: Q8N488; Q9P2W5; Q9UMW4
• Molecular Weight: 24,822 Da
• NCBI Official Full Name: RING1 and YY1-binding protein
• NCBI Official Synonym Full Names: RING1 and YY1 binding protein
• NCBI Official Symbol: RYBP
• NCBI Official Synonym Symbols: AAP1; DEDAF; YEAF1
• NCBI Protein Information: RING1 and YY1-binding protein; APAP-1; DED-associated factor; YY1 and E4TF1 associated factor 1; YY1 and E4TF1-associated factor 1; apoptin-associating protein 1; death effector domain-associated factor; ring1 interactor RYBP
• UniProt Protein Name: RING1 and YY1-binding protein
• Protein Family: RING1 and YY1-binding protein
• UniProt Gene Name: RYBP
• UniProt Synonym Gene Names: DEDAF; YEAF1; APAP-1; DED-associated factor
• UniProt Entry Name: RYBP_HUMAN

Uniprot Description

RYBP: Inhibits ubiquitination and subsequent degradation of TP53, and thereby plays a role in regulating transcription of TP53 target genes. May be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1. May bind to DNA. Promotes apoptosis.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 3p13

Cellular Component: nucleoplasm; cytoplasm; PcG protein complex

Molecular Function: protein binding; DNA binding; zinc ion binding; transcription corepressor activity

Biological Process: transcription, DNA-dependent; apoptosis; multicellular organismal development; negative regulation of transcription from RNA polymerase II promoter