Rabbit anti-Human RUSC2 Polyclonal Antibody | anti-RUSC2 antibody

RUSC2, NT (RUSC2, KIAA0375, Iporin, Interacting protein of Rab1, RUN and SH3 domain-containing protein 2) (Azide free) (HRP)

Cat. Num. AAA6344462

• Gene Names: RUSC2; MRT61; Iporin
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RUSC2; Polyclonal Antibody; NT (RUSC2; KIAA0375; Iporin; Interacting protein of Rab1; RUN and SH3 domain-containing protein 2) (Azide free) (HRP); RUN and SH3 domain-containing protein 2; anti-RUSC2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 1516

• Applicable Applications for anti-RUSC2 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: RUSC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 315-344 amino acids from the N-terminal region of human RUSC2.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RUSC2 Antibody (N-term) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the RUSC2 antibody detected the RUSC2 protein (arrow).)

Immunohistochemistry (IHC)

(RUSC2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RUSC2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-RUSC2 antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

Olsen, J.V., et al. Cell 127(3):635-648(2006). Olsen, J.V., et al. Cell 127(3):635-648(2006). Bayer, M., et al. BMC Cell Biol. 6 (1), 15 (2005).

NCBI and Uniprot Product Information

• NCBI GI #: 55741719
• NCBI GeneID: 9853
• NCBI Accession #: NP_055621.1; NP_001129471.1
• NCBI GenBank Nucleotide #: NP_055621.1
• UniProt Accession #: Q8N2Y8
• NCBI Official Full Name: iporin isoform 1
• NCBI Official Synonym Full Names: RUN and SH3 domain containing 2
• NCBI Official Symbol: RUSC2
• NCBI Official Synonym Symbols: MRT61; Iporin
• NCBI Protein Information: iporin
• UniProt Protein Name: Iporin
• Protein Family: Iporin
• UniProt Gene Name: RUSC2
• UniProt Synonym Gene Names: KIAA0375
• UniProt Entry Name: RUSC2_HUMAN

NCBI Description

This gene encodes a RUN and SH3 domain containing protein that interacts with Rab1b and Rab1-binding protein GM130. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jun 2012]

Uniprot Description

RUSC2: Interacts with active RAB1A and RAB1B, and with GOLGA2.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 9p13.3

Cellular Component: cytoplasmic vesicle; cytosol

Molecular Function: protein binding; Rab GTPase binding