Rabbit RUNX3 Polyclonal Antibody | anti-RUNX3 antibody

Rabbit anti RUNX3 Polyclonal

Cat. Num. AAA551091

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Serum IgG fraction was purified by Protein A affinity chromatography.
• Synonyms: RUNX3; Polyclonal Antibody; Rabbit anti RUNX3 Polyclonal; Rabbit anti RUNX3 Polyclonal purified; polyclonal; PAb; anti-RUNX3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Serum IgG fraction was purified by Protein A affinity chromatography.
• Form/Format: 0.2 um filtered solution in PBS with 5% trehalose.
• Sequence Length: 1298

• Applicable Applications for anti-RUNX3 antibody: Western Blot (WB), IHC (Immunohistochemistry), ELISA (EIA)
• Application Notes: ELISA: Use at a concentration range of 1-2ug/ml.; Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml.; IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml.; Immunohistochemical analysis of RUNX3 expression in paraffin fixed Human Liver carcinoma tissue.; It was used as primary antibody, dilution 1:400.
• Source Note: Polyclonal RUNX3 antibody was produced from sera of rabbits immunized with RUNX3 antigenic determinants.
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized antibody is stable for at least 1 year from date of receipt at -20 degree C.; Upon reconstitution, this antibody can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.; Shelf Life: >12 months

Immunohistochemistry

Product Categories/Family for anti-RUNX3 antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 103472027
• NCBI GeneID: 2324
• NCBI Accession #: NP_002011.2
• NCBI GenBank Nucleotide #: NM_002020.4
• UniProt Accession #: P35916; Q16067; Q86W07; Q86W08; A8K6L4; B5A926
• Molecular Weight: 152,757 Da
• NCBI Official Full Name: vascular endothelial growth factor receptor 3 isoform 2
• UniProt Protein Name: Vascular endothelial growth factor receptor 3
• Protein Family: Runt-related transcription factor
• UniProt Gene Name: FLT4
• UniProt Synonym Gene Names: VEGFR3; VEGFR-3; FLT-4
• UniProt Entry Name: VGFR3_HUMAN

NCBI Description

This gene encodes a tyrosine kinase receptor for vascular endothelial growth factors C and D. The protein is thought to be involved in lymphangiogenesis and maintenance of the lymphatic endothelium. Mutations in this gene cause hereditary lymphedema type IA. [provided by RefSeq, Jul 2008]

Uniprot Description

VEGFR3: Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFC and VEGFD, and plays an essential role in adult lymphangiogenesis and in the development of the vascular network and the cardiovascular system during embryonic development. Promotes proliferation, survival and migration of endothelial cells, and regulates angiogenic sprouting. Signaling by activated FLT4 leads to enhanced production of VEGFC, and to a lesser degree VEGFA, thereby creating a positive feedback loop that enhances FLT4 signaling. Modulates KDR signaling by forming heterodimers. The secreted isoform 3 may function as a decoy receptor for VEGFC and/or VEGFD and play an important role as a negative regulator of VEGFC-mediated lymphangiogenesis and angiogenesis. Binding of vascular growth factors to isoform 1 or isoform 2 leads to the activation of several signaling cascades; isoform 2 seems to be less efficient in signal transduction, because it has a truncated C-terminus and therefore lacks several phosphorylation sites. Mediates activation of the MAPK1/ERK2, MAPK3/ERK1 signaling pathway, of MAPK8 and the JUN signaling pathway, and of the AKT1 signaling pathway. Phosphorylates SHC1. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3- kinase. Promotes phosphorylation of MAPK8 at 'Thr-183' and 'Tyr- 185', and of AKT1 at 'Ser-473'. Interacts with VEGFC and VEGFD. Monomer in the absence of bound VEGFC or VEGFD. Homodimer in the presence of bound VEGFC or VEGFD. Can also form a heterodimer with KDR. Interacts with PTPN14; the interaction is enhanced by stimulation with VEGFC. Interacts with CRK, GRB2, PTK2/FAK1, SHC1, PIK3R1 and PTPN11/SHP- 2. Identified in a complex with SRC and ITGB1. Detected in endothelial cells. Widely expressed. Detected in fetal spleen, lung and brain. Detected in adult liver, muscle, thymus, placenta, lung, testis, ovary, prostate, heart, and kidney. Present in an inactive conformation in the absence of bound ligand. Binding of VEGFC or VEGFD leads to dimerization and activation by autophosphorylation on tyrosine residues. Inhibited by MAZ51. Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, tyrosine (receptor); Protein kinase, TK; Kinase, protein; Membrane protein, integral; EC 2.7.10.1; TK group; VEGFR family

Chromosomal Location of Human Ortholog: 5q35.3

Cellular Component: integral to plasma membrane; cytoplasm; plasma membrane; extracellular region; nucleus; receptor complex

Molecular Function: vascular endothelial growth factor receptor activity; protein binding; growth factor binding; transmembrane receptor protein tyrosine kinase activity; protein phosphatase binding; ATP binding

Biological Process: peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; positive regulation of JNK cascade; positive regulation of MAPKKK cascade; vasculature development; lymphangiogenesis; positive regulation of cell proliferation; blood vessel morphogenesis; positive regulation of endothelial cell proliferation; positive regulation of protein amino acid phosphorylation; sprouting angiogenesis; lymph vessel development; vascular endothelial growth factor receptor signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis

Disease: Hemangioma, Capillary Infantile; Lymphedema, Hereditary, Ia