Western Blot (WB)
((0. 05ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat RUFY1 Polyclonal Antibody | anti-RUFY1 antibody
Goat anti-RUFY1 Antibody
Gene Names
RUFY1; RABIP4; ZFYVE12
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
RUFY1; Polyclonal Antibody; Goat anti-RUFY1 Antibody; RUN and FYVE domain containing 1; FLJ22251; RABIP4; ZFYVE12; FYVE-finger protein EIP1; OTTHUMP00000223250; RUN and FYVE domain-containing 1; RUN and FYVE domain-containing protein 1; la-binding protein 1; rab4-interacting protein; zinc finger FYVE; RUFY1 antibody; RUN and FYVE domain containing 1 antibody; FLJ22251 antibody; RABIP4 antibody; ZFYVE12 antibody; FYVE-finger protein EIP1 antibody; OTTHUMP00000223250 antibody; RUN and FYVE domain-containing 1 antibody; RUN and FYVE domain-containing protein 1 antibody; rab4-interacting protein antibody; zinc finger FYVE antibody; anti-RUFY1 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
QKNEAITSFEGKT
Sequence Length
708
Applicable Applications for anti-RUFY1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 85kDa band observed in lysates of cell line HeLa (calculated MW of 79.8kDa according to NP_079434.3). Recommended concentration: 0. 05-0.2ug/ml.
Western Blot: Approx 85kDa band observed in lysates of cell line HeLa (calculated MW of 79.8kDa according to NP_079434.3). Recommended concentration: 0. 05-0.2ug/ml.
Immunogen
Peptide with sequence C-QKNEAITSFEGKT, from the internal region of the protein sequence according to NP_079434.3; NP_001035541.1.
Epitope
Internal region
Note
This antibody is expected to recognize both reported isoforms (NP_079434.3; NP_001035541.1). Reported variants represent identical protein: NP_001035542.1, NP_001035541.1.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0. 05ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0. 05ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
44,489 Da
NCBI Official Full Name
RUN and FYVE domain-containing protein 1 isoform a
NCBI Official Synonym Full Names
RUN and FYVE domain containing 1
NCBI Official Symbol
RUFY1
NCBI Official Synonym Symbols
RABIP4; ZFYVE12
NCBI Protein Information
RUN and FYVE domain-containing protein 1
UniProt Protein Name
RUN and FYVE domain-containing protein 1
Protein Family
UniProt Gene Name
RUFY1
UniProt Synonym Gene Names
RABIP4; ZFYVE12
UniProt Entry Name
RUFY1_HUMAN
NCBI Description
This gene encodes a protein that contains a RUN domain and a FYVE-type zinc finger domain. The encoded protein binds to phosphatidylinositol-3-phosphate (PI3P) and plays a role in early endosomal trafficking, tethering and fusion through interactions with small GTPases including Rab4, Rab5 and Rab14. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]
Uniprot Description
RUFY1: Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in early endosomal trafficking. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 5q35.3
Cellular Component: cytoplasm; early endosome membrane; intracellular membrane-bound organelle; nucleus
Molecular Function: lipid binding; protein binding; protein transporter activity; zinc ion binding
Biological Process: endocytosis; protein transport; regulation of endocytosis
Research Articles on RUFY1
Similar Products
Product Notes
The RUFY1 rufy1 (Catalog #AAA422549) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-RUFY1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's RUFY1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 85kDa band observed in lysates of cell line HeLa (calculated MW of 79.8kDa according to NP_079434.3). Recommended concentration: 0. 05-0.2ug/ml. Researchers should empirically determine the suitability of the RUFY1 rufy1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QKNEAITSFE GKT. It is sometimes possible for the material contained within the vial of "RUFY1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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