Rabbit RTN3 Polyclonal Antibody | anti-RTN3 antibody

Anti-RTN3 antibody

Cat. Num. AAA176986

• Gene Names: RTN3; HAP; ASYIP; NSPL2; NSPLII; RTN3-A1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified.
• Synonyms: RTN3; Polyclonal Antibody; Anti-RTN3 antibody; Reticulon-3; reticulon 3; ASYIP antibody; Neuroendocrine specific protein like 2 antibody; Neuroendocrine-specific protein-like 2 antibody; Neuroendocrine-specific protein-like II antibody; NSP like protein II antibody; NSP-like protein 2 antibody; NSP-like protein II antibody; NSPL2 antibody; NSPLII antibody; Reticulon 3 antibody; Reticulon-3 antibody; Reticulon3 antibody; Rtn3 antibody; RTN3_HUMAN antibody; anti-RTN3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 1032

• Applicable Applications for anti-RTN3 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human RTN3 (1017-1032aa EKIQAKLPGIAKKKAE), identical to the related mouse and rat sequences.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-RTN3 antibody, MBS176986, IHC(P)IHC(P): Rat Brain Tissue )

Immunohistochemistry (IHC)

(Anti-RTN3 antibody, MBS176986, IHC(P)IHC(P): Human Mammary Cancer Tissue )

Western Blot (WB)

(Anti-RTN3 antibody, MBS176986, Western blottingAll lanes: Anti RTN3 (MBS176986) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Mouse Brain Tissue Lysate at 50ugLane 3: U87 Whole Cell Lysate at 40ugPredicted bind size: 116KDObserved bind size: 150KD)

Related Product Information for anti-RTN3 antibody

Description: Rabbit IgG polyclonal antibody for Reticulon-3(RTN3) detection. Tested with WB, IHC-P in Human, Mouse, Rat.
Background: Reticulon-3, also known as RTN3 or NSPL2, is a protein that in humans is encoded by the RTN3 gene. It is mapped to 11q13, which is between markers D11S4535 and D11S4627. This gene belongs to the reticulon family of highly conserved genes that are preferentially expressed in neuroendocrine tissues. This family of proteins interact with, and modulate the activity of beta-amyloid converting enzyme 1(BACE1), and the production of amyloid-beta. RTN3 colocalized with BACE1 in neurons of human brain gray matter, and overexpression of RTN3 in vitro inhibited BACE1 activity and decreased amyloid precursor protein processing. 

NCBI and Uniprot Product Information

• NCBI GI #: 134047900
• NCBI GeneID: 10313
• UniProt Accession #: O95197; Q147U9; Q496K2; Q53GN3; Q59EP0; Q5UEP2; Q6T930; Q7RTM7; B3KQS2; B7Z308; B7Z4M0; F5H774
• Molecular Weight: 100,824 Da
• NCBI Official Full Name: Reticulon-3
• NCBI Official Synonym Full Names: reticulon 3
• NCBI Official Symbol: RTN3
• NCBI Official Synonym Symbols: HAP; ASYIP; NSPL2; NSPLII; RTN3-A1
• NCBI Protein Information: reticulon-3; NSP-like protein 2; NSP-like protein II; homolog of ASY protein; ASY interacting protein; neuroendocrine-specific protein-like 2; neuroendocrine-specific protein-like II
• UniProt Protein Name: Reticulon-3
• Protein Family: Reticulon
• UniProt Gene Name: RTN3
• UniProt Synonym Gene Names: ASYIP; NSPL2; HAP; NSP-like protein 2; NSP-like protein II; NSPLII
• UniProt Entry Name: RTN3_HUMAN

NCBI Description

This gene belongs to the reticulon family of highly conserved genes that are preferentially expressed in neuroendocrine tissues. This family of proteins interact with, and modulate the activity of beta-amyloid converting enzyme 1 (BACE1), and the production of amyloid-beta. An increase in the expression of any reticulon protein substantially reduces the production of amyloid-beta, suggesting that reticulon proteins are negative modulators of BACE1 in cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene, and pseudogenes of this gene are located on chromosomes 4 and 12. [provided by RefSeq, May 2012]

Uniprot Description

RTN3: May be involved in membrane trafficking in the early secretory pathway. Inhibits BACE1 activity and amyloid precursor protein processing. May induce caspase-8 cascade and apoptosis. May favor BCL2 translocation to the mitochondria upon endoplasmic reticulum stress. In case of enteroviruses infection, RTN3 may be involved in the viral replication or pathogenesis. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Endoplasmic reticulum; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: Golgi membrane; extracellular space; endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane

Biological Process: vesicle-mediated transport; viral reproduction; apoptosis