Western Blot (WB)
(RSBN1 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the RSBN1 antibody detected the RSBN1 protein (arrow).)
Rabbit anti-Human RSBN1 Polyclonal Antibody | anti-RSBN1 antibody
RSBN1, NT (RSBN1, Round spermatid basic protein 1) (Biotin)
Gene Names
RSBN1; KDM9; ROSBIN
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
RSBN1; Polyclonal Antibody; NT (RSBN1; Round spermatid basic protein 1) (Biotin); Round spermatid basic protein 1; anti-RSBN1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-RSBN1 antibody
Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
ELISA: 1:1,000
IHC: 1:10-50
WB: 1:100-500
Applications are based on unconjugated antibody.
ELISA: 1:1,000
IHC: 1:10-50
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
RSBN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 10-38 amino acids from the N-terminal region of human RSBN1.
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(RSBN1 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the RSBN1 antibody detected the RSBN1 protein (arrow).)
Western Blot (WB)
(RSBN1 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the RSBN1 antibody detected the RSBN1 protein (arrow).)
Immunohistochemistry (IHC)
(RSBN1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human plecenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RSBN1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(RSBN1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human plecenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RSBN1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(RSBN1 Antibody (N-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(RSBN1 Antibody (N-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-RSBN1 antibody
References
Rose, J. Phd, et al. Mol. Med. (2010). In press: Prahalad, S., et al. Arthritis Rheum. 60(7):2124-2130(2009). Cooper, J.D., et al. Nat. Genet. 40(12):1399-1401(2008). Raychaudhuri, S., et al. Nat. Genet. 40(10):1216-1223(2008). Smyth, D.J., et al. Diabetes 57(6):1730-1737(2008).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50,802 Da
NCBI Official Full Name
lysine-specific demethylase 9
NCBI Official Synonym Full Names
round spermatid basic protein 1
NCBI Official Symbol
RSBN1
NCBI Official Synonym Symbols
KDM9; ROSBIN
NCBI Protein Information
lysine-specific demethylase 9; round spermatid basic protein 1
UniProt Protein Name
Lysine-specific demethylase 9
Protein Family
UniProt Gene Name
RSBN1
UniProt Synonym Gene Names
KDM9
Uniprot Description
RSBN1: Belongs to the round spermatid basic protein 1 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Unknown function
Chromosomal Location of Human Ortholog: 1p13.2
Cellular Component: nucleus
Research Articles on RSBN1
Similar Products
Product Notes
The RSBN1 rsbn1 (Catalog #AAA6344196) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RSBN1, NT (RSBN1, Round spermatid basic protein 1) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RSBN1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 ELISA: 1:1,000 IHC: 1:10-50 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the RSBN1 rsbn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RSBN1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
