Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Western Blot (WB) (Western blot analysis of RS18 Antibody (Center) in mouse spleen tissue lysates (35ug/lane). RS18 (arrow) was detected using the purified Pab.)

Rabbit anti-Human, Mouse RS18 Polyclonal Antibody | anti-RS18 antibody

RS18, ID (40S Ribosomal Protein S18, Ke-3, Ke3, D6S218E) (AP)

Gene Names
RPS18; KE3; S18; HKE3; KE-3; D6S218E
Reactivity
Human, Mouse
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
RS18; Polyclonal Antibody; ID (40S Ribosomal Protein S18; Ke-3; Ke3; D6S218E) (AP); anti-RS18 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human RS18. Species Crossreactivity: mouse.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-RS18 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:50-1:100
ELISA: 1:1000
WB: 1:100-1:500
Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide corresponding to a portion of amino acids within aa69-99 from the center region of human RS18.
Conjugate
AP
Note
Preservative Free
Preparation and Storage
May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of RS18 Antibody (Center) in mouse spleen tissue lysates (35ug/lane). RS18 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of RS18 Antibody (Center) in mouse spleen tissue lysates (35ug/lane). RS18 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human colon carcinoma reacted with RS18 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human colon carcinoma reacted with RS18 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Product Categories/Family for anti-RS18 antibody
References
1. Yu, Y., Ji, H., et.al., Protein Sci. 14(6), 1438-1446 (2005).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
17,719 Da
NCBI Official Full Name
40S ribosomal protein S18
NCBI Official Synonym Full Names
ribosomal protein S18
NCBI Official Symbol
RPS18
NCBI Official Synonym Symbols
KE3; S18; HKE3; KE-3; D6S218E
NCBI Protein Information
40S ribosomal protein S18; rhabdomyosarcoma antigen MU-RMS-40.21
UniProt Protein Name
40S ribosomal protein S18
UniProt Gene Name
RPS18
UniProt Synonym Gene Names
D6S218E; Ke3
UniProt Entry Name
RS18_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S13P family of ribosomal proteins. It is located in the cytoplasm. The gene product of the E. coli ortholog (ribosomal protein S13) is involved in the binding of fMet-tRNA, and thus, in the initiation of translation. This gene is an ortholog of mouse Ke3. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Uniprot Description

RPS18: Located at the top of the head of the 40S subunit, it contacts several helices of the 18S rRNA. Belongs to the ribosomal protein S13P family.

Protein type: Translation; Ribosomal

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: small ribosomal subunit; focal adhesion; membrane; cytoplasm; ribosome; nucleus; cytosol

Molecular Function: rRNA binding; protein binding; structural constituent of ribosome

Biological Process: SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; translational elongation; translation; viral reproduction; mRNA catabolic process, nonsense-mediated decay; translational initiation; ribosome biogenesis and assembly; viral transcription; gene expression; viral infectious cycle; translational termination

Research Articles on RS18

Similar Products

Product Notes

The RS18 rps18 (Catalog #AAA6497751) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RS18, ID (40S Ribosomal Protein S18, Ke-3, Ke3, D6S218E) (AP) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RS18 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-1:100 ELISA: 1:1000 WB: 1:100-1:500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the RS18 rps18 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RS18, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.