Rabbit anti-Human RRN3 Polyclonal Antibody | anti-RRN3 antibody

RRN3 (Phospho-S649) Polyclonal Antibody

Cat. Num. AAA3007942

• Gene Names: RRN3; TIFIA; A-270G1.2
• Reactivity: Human
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: RRN3; Polyclonal Antibody; RRN3 (Phospho-S649) Polyclonal Antibody; RNA polymerase I-specific transcription initiation factor RRN3; Transcription initiation factor IA; TIF-IA; TIFIA; anti-RRN3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: RRN3 (phospho-S649) polyclonal antibody detects endogenous levels of RRN3 protein only when phosphorylated at Ser649.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.3.
• Sequence Length: 651

• Applicable Applications for anti-RRN3 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic phosphopeptide derived from human RRN3 around the phosphorylation site of Serine649.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of RRN3 (phospho-S649) polyclonal antibody at 1:500 dilutionLane1:A375 whole cell lysate (40ug) Lane2:EC9706 whole cell lysate (40ug) Lane3:SGC7901 whole cell lysate (40ug))

Related Product Information for anti-RRN3 antibody

In eukaryotes, ribosomal RNA genes are transcribed by RNA polymerase (pol I). In Saccharomyces cerevisiae, transcription of rRNA genes requires at least three transcription factors, which include the two multisubunit factors Core factor and UAF that function in the assembly of the preinitiation complex. The third factor, Rrn3, functions as a single subunit and is not required for the preinitiation complex assembly. Unlike other pol I transcription factors, Rrn3 is functionally conserved between yeast and mammals as an rRNA gene transcription regulator. Human Rrn3 is 21% homologous to the yeast Rrn3 protein and is a member of a conserved gene family spanning the fungi, plant and animal kingdoms. hRrn3 is highly expressed in the lung, retina, thymus, and prostate. Rrn3 may be identical to the transcription factor TIF-IA, since both TIF-IA and Rrn3 associate with pol I and their activities are growth rate dependent.

NCBI and Uniprot Product Information

• NCBI GI #: 93102377
• NCBI GeneID: 54700
• NCBI Accession #: NP_060897.3
• NCBI GenBank Nucleotide #: NP_060897.3
• UniProt Accession #: Q9NYV6
• Molecular Weight: ~ 80kDa
• NCBI Official Full Name: RNA polymerase I-specific transcription initiation factor RRN3 isoform 1
• NCBI Official Synonym Full Names: RRN3 homolog, RNA polymerase I transcription factor
• NCBI Official Symbol: RRN3
• NCBI Official Synonym Symbols: TIFIA; A-270G1.2
• NCBI Protein Information: RNA polymerase I-specific transcription initiation factor RRN3
• UniProt Protein Name: RNA polymerase I-specific transcription initiation factor RRN3
• Protein Family: RNA polymerase I-specific transcription initiation factor
• UniProt Gene Name: RRN3
• UniProt Synonym Gene Names: TIFIA; TIF-IA
• UniProt Entry Name: RRN3_HUMAN

Uniprot Description

TIF1A: a transcription initiation factor. RNA polymerase I transcription factor RRN3. A growth-dependent regulator of ribosomal RNA synthesis

Protein type: Transcription initiation complex; Nucleolus

Chromosomal Location of Human Ortholog: 16p12

Cellular Component: nucleoplasm; nucleolus; nucleus

Biological Process: cell proliferation; in utero embryonic development; positive regulation of transcription, DNA-dependent; ribosome biogenesis and assembly; homeostasis of number of cells; transcription from RNA polymerase I promoter; gene expression; cytoplasm organization and biogenesis; transcription initiation from RNA polymerase I promoter; nucleolus organization and biogenesis