Rabbit anti-Human, Mouse RRAGB Polyclonal Antibody | anti-RRAGB antibody

RRAGB (Ras-related GTP-binding Protein B, Rag B, RagB) (Biotin)

Cat. Num. AAA6393004

• Gene Names: RRAGB; RAGB; bA465E19.1
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RRAGB; Polyclonal Antibody; RRAGB (Ras-related GTP-binding Protein B; Rag B; RagB) (Biotin); anti-RRAGB antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RRAGB. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-RRAGB antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full-length human RRAGB, aa1-374 (NP_057740.2).
• Immunogen Sequence: MEESDSEKTTEKENLGPRMDPPLGEPEGSLGWVLPNTAMKKKVLLMGKSGSGKTSMRSIIFANYIARDTRRLGATILDRIHSLQINSSLSTYSLVDSVGNTKTFDVEHSHVRFLGNLVLNLWDCGGQDTFMENYFTSQRDNIFRNVEVLIYVFDVESRELEKDMHYYQSCLEAILQNSPDAKIFCLVHKMDLVQEDQRDLIFKEREEDLRRLSRPLECSCFRTSIWDETLYKAWSSIVYQLIPNVQQLEMNLRNFAEIIEADEVLLFERATFLVISHYQCKEQRDAHRFEKISNIIKQFKLSCSKLAASFQSMEVRNSNFAAFIDIFTSNTYVMVVMSDPSIPSAATLINIRNARKHFEKLERVDGPKQCLLMR
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RRAGB rabbit polyclonal antibody. Western Blot analysis of RRAGB expression in human placenta.)

Western Blot (WB)

(RRAGB rabbit polyclonal antibody. Western Blot analysis of RRAGB expression in mouse brain.)

Western Blot (WB)

(RRAGB rabbit polyclonal antibody. Western Blot analysis of RRAGB expression in A-431.)

Western Blot (WB)

(Western Blot analysis of RRAGB expression in transfected 293T cell line by RRAGB polyclonal antibody. Lane 1: RRAGB transfected lysate (43.3kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-RRAGB antibody

Ras-homologous GTPases constitute a large family of signal transducers that alternate between an activated, GTP-binding state and an inactivated, GDP-binding state. These proteins represent cellular switches that are operated by GTP-exchange factors and factors that stimulate their intrinsic GTPase activity. All GTPases of the Ras superfamily have in common the presence of six conserved motifs involved in GTP/GDP binding, three of which are phosphate-/magnesium-binding sites (PM1-PM3) and three of which are guanine nucleotide-binding sites (G1-G3). Transcript variants encoding distinct isoforms have been identified. [provided by RefSeq].

Product Categories/Family for anti-RRAGB antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 46249397
• NCBI GeneID: 10325
• NCBI Accession #: NP_057740.2
• NCBI GenBank Nucleotide #: NM_016656.2
• UniProt Accession #: Q5VZM2; Q15348; Q8N3T0; A8K042
• Molecular Weight: 43,250 Da
• NCBI Official Full Name: ras-related GTP-binding protein B long isoform
• NCBI Official Synonym Full Names: Ras-related GTP binding B
• NCBI Official Symbol: RRAGB
• NCBI Official Synonym Symbols: RAGB; bA465E19.1
• NCBI Protein Information: ras-related GTP-binding protein B; rag B; GTP-binding protein ragB
• UniProt Protein Name: Ras-related GTP-binding protein B
• Protein Family: Ras-related GTP-binding protein
• UniProt Gene Name: RRAGB
• UniProt Synonym Gene Names: Rag B; RagB
• UniProt Entry Name: RRAGB_HUMAN

NCBI Description

Ras-homologous GTPases constitute a large family of signal transducers that alternate between an activated, GTP-binding state and an inactivated, GDP-binding state. These proteins represent cellular switches that are operated by GTP-exchange factors and factors that stimulate their intrinsic GTPase activity. All GTPases of the Ras superfamily have in common the presence of six conserved motifs involved in GTP/GDP binding, three of which are phosphate-/magnesium-binding sites (PM1-PM3) and three of which are guanine nucleotide-binding sites (G1-G3). Transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

RRAGB: Has guanine nucleotide-binding activity but undetectable intrinsic GTPase activity. Required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids. Involved in the RCC1/Ran-GTPase pathway. Involved in the RCC1/Ran-GTPase pathway. The short isoform binds GTP. Interacts with RRAGC and RRAGD. Belongs to the GTR/RAG GTP-binding protein family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: Xp11.21

Cellular Component: Golgi apparatus; intracellular membrane-bound organelle; lysosomal membrane; lysosome; cytoplasm; nucleus

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding; guanyl ribonucleotide binding

Biological Process: metabolic process; regulation of autophagy; cellular response to starvation; positive regulation of TOR signaling pathway