Immunohistochemistry (IHC)
(Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE))
Rabbit RPS6KB1/S6K RPS6KB1 Polyclonal Antibody | anti-RPS6KB1 antibody
RPS6KB1/S6K RPS6KB1 (STK14A, Ribosomal protein S6 kinase beta-1, 70kD ribosomal protein S6 kinase 1, Ribosomal protein S6 kinase I, p70 ribosomal S6 kinase alpha)
Purified by immunoaffinity chromatography
Purified by immunoaffinity chromatography
Dilution: ELISA: 1:5000
Western Blot: 1:500-1:1000
Immunohistochemistry (Paraffin): 10ug/ml
Immunohistochemistry (IHC)
(Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunohistochemistry (IHC)
(Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE))
Western Blot (WB)
(Western blot analysis of extracts from COLO205/Jurkat cells, using S6K. The lane on the right is treated with the synthesized peptide.)
Western Blot (WB)
(Western blot analysis of extracts from COLO205/Jurkat cells, using S6K. The lane on the right is treated with the synthesized peptide.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. [provided by RefSeq, Jan 2013]
Uniprot Description
Function: Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the preinitiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. Also controls translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, targeting it for ubiquitination and subsequent proteolysis. Promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR. In response to IGF1, activates translation elongation by phosphorylating EEF2 kinase (EEF2K), which leads to its inhibition and thus activation of EEF2. Also plays a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating RICTOR, resulting in the inhibition of mTORC2 and AKT1 signaling. Mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function. Phosphorylates mitochondrial URI1 leading to dissociation of a URI1-PPP1CC complex. The free mitochondrial PPP1CC can then dephosphorylate RPS6KB1 at 'Thr-412', which is proposed to be a negative feedback mechanism for the RPS6KB1 anti-apoptotic function. Mediates TNF-alpha-induced insulin resistance by phosphorylating IRS1 at multiple serine residues, resulting in accelerated degradation of IRS1. In cells lacking functional TSC1-2 complex, constitutively phosphorylates and inhibits GSK3B. May be involved in cytoskeletal rearrangement through binding to neurabin. Ref.4 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.14 Ref.16 Ref.18 Ref.20 Ref.23 Ref.24
Catalytic activity: ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation: Activation requires multiple phosphorylation events on serine/threonine residues. Activation appears to be first mediated by phosphorylation of multiple sites in the autoinhibitory domain, which facilitates phosphorylation at Thr-412, disrupting the autoinhibitory mechanism and allowing phosphorylation of Thr-252 by PDPK1. The active conformation of the kinase is believed to be stabilized by a mechanism involving three conserved phosphorylation sites located in the kinase domain activation loop (Thr-252) and in the AGC-kinase C-terminal domain (Ser-394 in the middle of the tail/linker region and Thr-412 within a hydrophobic motif at its end). Activated by mTORC1; isoform Alpha I and isoform Alpha II are sensitive to rapamycin, which inhibits activating phosphorylation at Thr-412. Activated by PDPK1. Ref.3 Ref.13 Ref.19
Subunit structure: Interacts with PPP1R9A/neurabin-1
By similarity. Interacts with RPTOR. Interacts with IRS1. Interacts with EIF3B and EIF3C. Interacts with POLDIP3 and TRAF4. Ref.5 Ref.6 Ref.7 Ref.10 Ref.16
Subcellular location: Cell junction › synapse › synaptosome
By similarity. Mitochondrion outer membrane. Mitochondrion. Note: Colocalizes with URI1 at mitochondrion. Ref.14 Ref.18Isoform Alpha I: Nucleus. Cytoplasm Ref.14 Ref.18. Isoform Alpha II: Cytoplasm Ref.14 Ref.18.
Tissue specificity: Widely expressed. Ref.2
Domain: The autoinhibitory domain is believed to block phosphorylation within the AGC-kinase C-terminal domain and the activation loop.The TOS (TOR signaling) motif is essential for activation by mTORC1
By similarity.
Post-translational modification: Phosphorylation at Thr-412 is regulated by mTORC1. The phosphorylation at this site is maintained by an agonist-dependent autophosphorylation mechanism
By similarity. Activated by phosphorylation at Thr-252 by PDPK1. Dephosphorylation by PPP1CC at Thr-412 in mitochondrion.
Sequence similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.Contains 1 AGC-kinase C-terminal domain.Contains 1 protein kinase domain.
Research Articles on RPS6KB1
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Product Notes
The RPS6KB1 rps6kb1 (Catalog #AAA649515) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RPS6KB1/S6K RPS6KB1 (STK14A, Ribosomal protein S6 kinase beta-1, 70kD ribosomal protein S6 kinase 1, Ribosomal protein S6 kinase I, p70 ribosomal S6 kinase alpha) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RPS6KB1/S6K RPS6KB1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot, Immunohistochemistry. Dilution: ELISA: 1:5000 Western Blot: 1:500-1:1000 Immunohistochemistry (Paraffin): 10ug/ml. Researchers should empirically determine the suitability of the RPS6KB1 rps6kb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPS6KB1/S6K RPS6KB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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