Rabbit RPS6KA5 Polyclonal Antibody | anti-RPS6KA5 antibody

RPS6KA5 Antibody

Cat. Num. AAA7049060

• Gene Names: RPS6KA5; MSK1; RLPK; MSPK1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: RPS6KA5; Polyclonal Antibody; RPS6KA5 Antibody; Ribosomal protein S6 kinase alpha-5; S6K-alpha-5; 90 kDa ribosomal protein S6 kinase 5; Nuclear mitogen- and stress-activated protein kinase 1; RSK-like protein kinase; RSKL; MSK1; anti-RPS6KA5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 723

• Applicable Applications for anti-RPS6KA5 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1: 500-1: 5000; IHC: 1: 20-1: 200; IF: 1: 50-1: 200
• Immunogen: Recombinant Human Ribosomal protein S6 kinase alpha-5 protein (224-367AA)
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Conjugation: Non-conjugated
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-25417 / sc-9391 / sc-9392
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: Mouse brain tissue,Rat heart tissue,Rat liver tissueAll lanes: RPS6KA5 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 90,62,82 KDaObserved band size: 90 KDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human thyroid tissue using MBS7049060 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human placenta tissue using MBS7049060 at dilution of 1:100)

Immunofluorescence (IF)

(Immunofluorescent analysis of MCF-7 cells using MBS7049060 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-RPS6KA5 antibody

Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factors RELA, STAT3 and ETV1/ER81, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin. Plays an essential role in the control of RELA transcriptional activity in response to TNF and upon glucocorticoid, associates in the cytoplasm with the glucocorticoid receptor NR3C1 and contributes to RELA inhibition and repression of inflammatory gene expression. In skeletal myoblasts is required for phosphorylation of RELA at 'Ser-276' during oxidative stress. In erythropoietin-stimulated cells, is necessary for the 'Ser-727' phosphorylation of STAT3 and regulation of its transcriptional potential. Phosphorylates ETV1/ER81 at 'Ser-191' and 'Ser-216', and thereby regulates its ability to stimulate transcription, which may be important during development and breast tumor formation. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines. Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors. Plays a role in neuronal cell death by mediating the downstream effects of excitotoxic injury.

NCBI and Uniprot Product Information

• NCBI GI #: 1016080537
• NCBI GeneID: 9252
• NCBI Accession #: NP_001309164.1
• NCBI GenBank Nucleotide #: NM_001322235.1
• UniProt Accession #: O75582; O95316; Q96AF7; B7Z2Y5
• Molecular Weight: 81,780 Da
• NCBI Official Full Name: ribosomal protein S6 kinase alpha-5 isoform k
• NCBI Official Synonym Full Names: ribosomal protein S6 kinase A5
• NCBI Official Symbol: RPS6KA5
• NCBI Official Synonym Symbols: MSK1; RLPK; MSPK1
• NCBI Protein Information: ribosomal protein S6 kinase alpha-5
• UniProt Protein Name: Ribosomal protein S6 kinase alpha-5
• Protein Family: Ribosomal protein S6 kinase
• UniProt Gene Name: RPS6KA5
• UniProt Synonym Gene Names: MSK1; S6K-alpha-5; RSKL
• UniProt Entry Name: KS6A5_HUMAN

Uniprot Description

MSK1: an AGC kinase of the RSK family. Has two kinase domains connected by a regulatory linker region. Enzyme activity requires the presence of both kinase domains. Is phosphorylated and activated by Erk as well as p38 MAPK in response to growth factors and cellular stress.

Protein type: AGC group; EC 2.7.11.1; Kinase, protein; MSK subfamily; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); RSK family

Chromosomal Location of Human Ortholog: 14q31-q32.1

Cellular Component: cytoplasm; nucleoplasm; nucleus

Molecular Function: ATP binding; histone serine kinase activity (H3-S10 specific); protein binding; protein kinase activity; protein serine/threonine kinase activity

Biological Process: activation of CREB transcription factor; activation of NF-kappaB transcription factor; axon guidance; epidermal growth factor receptor signaling pathway; histone phosphorylation; negative regulation of cytokine production; negative regulation of transcription, DNA-dependent; positive regulation of histone acetylation; positive regulation of histone phosphorylation; positive regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; regulation of transcription, DNA-dependent; stimulatory C-type lectin receptor signaling pathway