Rabbit anti-Human RPS6KA1 / RSK1 Polyclonal Antibody | anti-RPS6KA1 antibody

Rabbit Polyclonal to Human RPS6KA1 / RSK1

Cat. Num. AAA241313

• Gene Names: RPS6KA1; RSK; HU-1; RSK1; p90Rsk; MAPKAPK1A
• Reactivity: Human
• Applications: Immunohistochemistry, Immunocytochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: RPS6KA1 / RSK1; Polyclonal Antibody; Rabbit Polyclonal to Human RPS6KA1 / RSK1; Anti-RPS6KA1 / RSK1 Antibody (N-Terminus) IHC-plus; RPS6KA1; MAPKAPK-1a; p90RSK; p90-RSK 1; Pp90RSK1; Ribosomal S6 kinase 1; RSK-1; S6K-alpha 1; MAPKAP kinase 1a; MAPKAPK1A; p90 s6 kinase; p90S6K; RSK; RSK1; HU-1; p90RSK1; S6K-alpha-1; Human RPS6KA1; anti-RPS6KA1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: 16 amino acid peptide from near the amino-terminus of human RPS6K1
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 735

• Applicable Applications for anti-RPS6KA1 antibody: Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB)
• Application Notes: ICC, IHC-P (5 ug/ml), WB (5 - 10 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human RPS6KA1 / RSK1.
• Immunogen Type: Synthetic peptide
• Immunogen: RPS6KA1 / RSK1 antibody was raised against synthetic peptide from human RPS6KA1 / RSK1.
• Antigen Modification: N-Terminus
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-RPS6KA1 / RSK1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of RP S6 Kinase in Jurkat cells with RP S6 Kinase antibody at 20 ug/ml.)

Western Blot (WB)

(Western blot of RPS6K1 in Jurkat cell lysate with RPS6K1 antibody at (A) 2.5, (B) 5 and (C) 10 ug/ml.)

Related Product Information for anti-RPS6KA1 antibody

RSK1 is an RSK serine/threonine kinase. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Product Categories/Family for anti-RPS6KA1 antibody

Family: Protein Kinase; Subfamily: RSK

NCBI and Uniprot Product Information

• NCBI GI #: 20149547
• NCBI GeneID: 6195
• NCBI Accession #: NP_002944.2
• NCBI GenBank Nucleotide #: NM_002953.3
• UniProt Accession #: Q15418; Q5SVM5; Q5SVM8; Q5SVM9; Q96C05; Q9BQK2; A6NGG4; A8K9K7; B2RDY8; B7Z5J0
• Molecular Weight: 72,698 Da
• NCBI Official Full Name: ribosomal protein S6 kinase alpha-1 isoform a
• NCBI Official Synonym Full Names: ribosomal protein S6 kinase, 90kDa, polypeptide 1
• NCBI Official Symbol: RPS6KA1
• NCBI Official Synonym Symbols: RSK; HU-1; RSK1; p90Rsk; MAPKAPK1A
• NCBI Protein Information: ribosomal protein S6 kinase alpha-1; RSK-1; p90S6K; p90RSK1; p90-RSK 1; MAPKAPK-1a; S6K-alpha 1; S6K-alpha-1; MAPKAP kinase 1a; ribosomal S6 kinase 1; MAPK-activated protein kinase 1a; ribosomal protein S6 kinase alpha 1; 90 kDa ribosomal protein S6 kinase 1; MAP kinase-activated protein kinase 1a; dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1)
• UniProt Protein Name: Ribosomal protein S6 kinase alpha-1
• Protein Family: Ribosomal protein S6 kinase
• UniProt Gene Name: RPS6KA1
• UniProt Synonym Gene Names: MAPKAPK1A; RSK1; S6K-alpha-1; p90-RSK 1; p90RSK1; p90S6K; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a; RSK-1
• UniProt Entry Name: KS6A1_HUMAN

NCBI Description

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

p90RSK: an AGC kinase of the RSK family. Phosphorylated and activated by Erk1 and -2 in response to many growth factors, polypeptide hormones and neurotransmitters. Several phosphorylation sites are important for its activation. Possesses two kinase domains connected by a regulator linker region. Phosphorylates a wide range of substrates including ribosomal protein S6. Prominently expressed in brain structures essential for cognitive function and learning.

Protein type: Kinase, protein; Translation; EC 2.7.11.1; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); AGC group; RSK family; RSK subfamily

Chromosomal Location of Human Ortholog: 1p

Cellular Component: nucleoplasm; cytoplasm; spindle; nucleus; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; caspase inhibitor activity; magnesium ion binding; protein serine/threonine/tyrosine kinase activity; ATP binding

Biological Process: regulation of transcription in response to stress; axon guidance; nerve growth factor receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; regulation of translation in response to stress; negative regulation of caspase activity; toll-like receptor 3 signaling pathway; signal transduction; cell cycle; positive regulation of cell growth; protein amino acid phosphorylation; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; synaptic transmission; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; positive regulation of cell differentiation; toll-like receptor 4 signaling pathway; negative regulation of apoptosis