Western Blot (WB)
(Western blot analysis of RPS6 (pS235) expression in HeLa Calyculin A-treated (A), NIH3T3 (B) whole cell lysates.)
Rabbit RPS6 Polyclonal Antibody | anti-RPS6 antibody
Anti-RPS6 (pS235) Phospho Antibody
Western Blot (WB)
(Western blot analysis of RPS6 (pS235) expression in HeLa Calyculin A-treated (A), NIH3T3 (B) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of RPS6 (pS235) expression in HeLa Calyculin A-treated (A), NIH3T3 (B) whole cell lysates.)
Immunofluorescence (IF)
(Immunofluorescent analysis of RPS6 (pS235) staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Immunofluorescence (IF)
(Immunofluorescent analysis of RPS6 (pS235) staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
NCBI and Uniprot Product Information
NCBI Description
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
Uniprot Description
S6: May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. Belongs to the ribosomal protein S6e family.
Protein type: Translation
Chromosomal Location of Human Ortholog: 9p21
Cellular Component: polysome; small ribosomal subunit; membrane; perinuclear region of cytoplasm; dendrite; cytoplasm; nucleolus; cytosol; nucleus; ribonucleoprotein complex
Molecular Function: protein binding; structural constituent of ribosome; protein kinase binding
Biological Process: SRP-dependent cotranslational protein targeting to membrane; erythrocyte development; TOR signaling pathway; translation; viral reproduction; positive regulation of apoptosis; T cell differentiation in the thymus; gastrulation; viral infectious cycle; translational termination; glucose homeostasis; oogenesis stage; T cell proliferation during immune response; translational initiation; ribosomal small subunit assembly and maintenance; viral transcription; activated T cell apoptosis; placenta development; mitosis; mitotic cell cycle checkpoint; ribosomal small subunit biogenesis and assembly; cellular protein metabolic process; translational elongation; insulin receptor signaling pathway; mRNA catabolic process, nonsense-mediated decay; gene expression; rRNA processing; G1/S transition of mitotic cell cycle; negative regulation of apoptosis
Research Articles on RPS6
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Product Notes
The RPS6 rps6 (Catalog #AAA8226102) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RPS6 (pS235) Phospho Antibody reacts with Human, Mouse, Rat, Bovine, Chicken, Monkey, Sheep, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's RPS6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC). WB (1/500 - 1/1000), IF (1/100 - 1/500), ICC (1/100 - 1/500). Researchers should empirically determine the suitability of the RPS6 rps6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPS6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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