Rabbit anti-Human RPS4Y1 Polyclonal Antibody | anti-RPS4Y1 antibody

RPS4Y1, ID (RPS4Y1, RPS4Y, 40S ribosomal protein S4, Y isoform 1) (AP)

Cat. Num. AAA6343864

• Gene Names: RPS4Y1; S4; RPS4Y
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RPS4Y1; Polyclonal Antibody; ID (RPS4Y1; RPS4Y; 40S ribosomal protein S4; Y isoform 1) (AP); Y isoform 1; anti-RPS4Y1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RPS4Y1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-RPS4Y1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 83-113 amino acids from the Central region of human RPS4Y1 (NP_000999.1). Species sequence homology: Chicken, Xenopus
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in K562 cell line lysates (35ug/lane) usingMBS6010278.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining usingMBS6010278.)

Flow Cytometry (FC/FACS)

(Flow Cytometry analysis of K562 cells using MBS6010278 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-RPS4Y1 antibody

Cytoplasmic ribosomes, organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes ribosomal protein S4, a component of the 40S subunit. Ribosomal protein S4 is the only ribosomal protein known to be encoded by more than one gene, namely this gene and ribosomal protein S4, X-linked (RPS4X). The 2 isoforms encoded by these genes are not identical, but are functionally equivalent. Ribosomal protein S4 belongs to the S4E family of ribosomal proteins. It has been suggested that haploinsufficiency of the ribosomal protein S4 genes plays a role in Turner syndrome; however, this hypothesis is controversial. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Product Categories/Family for anti-RPS4Y1 antibody

Antibodies; Ribosomal Proteins

References

1. Agate, R.J., et al. Mol. Biol. Evol. 21(2):384-396(2004). 2. Vawter, M.P., et al. Neuropsychopharmacology 29(2):373-384(2004). 3. Kapp, L.D., et al. Annu. Rev. Biochem. 73, 657-704 (2004) :4. Skaletsky, H., et al. Nature 423(6942):825-837(2003). 5. Sampath, P., et al. Mol. Cell. Biol. 23(5):1509-1519(2003)

NCBI and Uniprot Product Information

• NCBI GI #: 4506727
• NCBI GeneID: 6192
• NCBI Accession #: NP_000999.1
• NCBI GenBank Nucleotide #: NM_001008.3
• UniProt Accession #: P22090; A8K9V4
• Molecular Weight: 29,456 Da
• NCBI Official Full Name: 40S ribosomal protein S4, Y isoform 1
• NCBI Official Synonym Full Names: ribosomal protein S4, Y-linked 1
• NCBI Official Symbol: RPS4Y1
• NCBI Official Synonym Symbols: S4; RPS4Y
• NCBI Protein Information: 40S ribosomal protein S4, Y isoform 1; ribosomal protein S4Y
• UniProt Protein Name: 40S ribosomal protein S4, Y isoform 1
• Protein Family: 40S ribosomal protein
• UniProt Gene Name: RPS4Y1
• UniProt Synonym Gene Names: RPS4Y
• UniProt Entry Name: RS4Y1_HUMAN

NCBI Description

Cytoplasmic ribosomes, organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes ribosomal protein S4, a component of the 40S subunit. Ribosomal protein S4 is the only ribosomal protein known to be encoded by more than one gene, namely this gene and ribosomal protein S4, X-linked (RPS4X). The 2 isoforms encoded by these genes are not identical, but are functionally equivalent. Ribosomal protein S4 belongs to the S4E family of ribosomal proteins. It has been suggested that haploinsufficiency of the ribosomal protein S4 genes plays a role in Turner syndrome; however, this hypothesis is controversial. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Uniprot Description

RPS4Y1: Cytoplasmic ribosomes, organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes ribosomal protein S4, a component of the 40S subunit. Ribosomal protein S4 is the only ribosomal protein known to be encoded by more than one gene, namely this gene and ribosomal protein S4, X-linked (RPS4X). The 2 isoforms encoded by these genes are not identical, but are functionally equivalent. Ribosomal protein S4 belongs to the S4E family of ribosomal proteins. It has been suggested that haploinsufficiency of the ribosomal protein S4 genes plays a role in Turner syndrome; however, this hypothesis is controversial. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Protein type: Translation; Ribosomal

Chromosomal Location of Human Ortholog: Yp11.3

Cellular Component: polysome; membrane; cytosol; nucleus

Molecular Function: rRNA binding; structural constituent of ribosome; RNA binding

Biological Process: SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; translational elongation; viral reproduction; translation; multicellular organismal development; translational initiation; mRNA catabolic process, nonsense-mediated decay; viral transcription; gene expression; viral infectious cycle; translational termination