Rabbit anti-Human RPS24 Polyclonal Antibody | anti-RPS24 antibody

RPS24, ID (RPS24, 40S ribosomal protein S24) (APC)

Cat. Num. AAA6343821

• Gene Names: RPS24; S24; DBA3
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RPS24; Polyclonal Antibody; ID (RPS24; 40S ribosomal protein S24) (APC); 40S ribosomal protein S24; anti-RPS24 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-RPS24 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: RPS24 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 72~101 amino acids from the Central region of human RPS24.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of RPS24 Antibody (Center) in MCF-7 cell line lysates (35ug/lane). RPS24 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(RPS24 Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-RPS24 antibody

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPS24 is a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S24E family of ribosomal proteins. It is located in the cytoplasm. Multiple transcript variants encoding different isoforms have been found for this gene. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Product Categories/Family for anti-RPS24 antibody

Antibodies; Ribosomal Proteins

References

Quarello, P., et al. Haematologica 95(2):206-213(2010). Badhai, J., et al. Biochim. Biophys. Acta 1792(10):1036-1042(2009). Robledo, S., et al. RNA 14(9):1918-1929(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4506703
• NCBI GeneID: 6229
• NCBI Accession #: NP_001017.1
• NCBI GenBank Nucleotide #: NM_001026.4
• UniProt Accession #: P62847; P16632; Q5T0P7; Q5T0P8; Q7Z3D1; E7EPK6
• Molecular Weight: 32,431 Da
• NCBI Official Full Name: 40S ribosomal protein S24 isoform c
• NCBI Official Synonym Full Names: ribosomal protein S24
• NCBI Official Symbol: RPS24
• NCBI Official Synonym Symbols: S24; DBA3
• NCBI Protein Information: 40S ribosomal protein S24
• UniProt Protein Name: 40S ribosomal protein S24
• Protein Family: 40S ribosomal protein
• UniProt Gene Name: RPS24
• UniProt Entry Name: RS24_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S24E family of ribosomal proteins. It is located in the cytoplasm. Multiple transcript variants encoding different isoforms have been found for this gene. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Mutations in this gene result in Diamond-Blackfan anemia. [provided by RefSeq, Nov 2008]

Uniprot Description

RPS24: Required for processing of pre-rRNA and maturation of 40S ribosomal subunits. Defects in RPS24 are the cause of Diamond-Blackfan anemia type 3 (DBA3). DBA3 is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies. Belongs to the ribosomal protein S24e family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Translation; Ribosomal

Chromosomal Location of Human Ortholog: 10q22

Cellular Component: small ribosomal subunit; membrane; cytoplasm; nucleolus; nucleus; cytosol

Molecular Function: structural constituent of ribosome; translation initiation factor binding; nucleotide binding

Biological Process: SRP-dependent cotranslational protein targeting to membrane; viral reproduction; translation; viral infectious cycle; translational termination; ribosomal small subunit biogenesis and assembly; erythrocyte homeostasis; maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); cellular protein metabolic process; translational elongation; translational initiation; mRNA catabolic process, nonsense-mediated decay; gene expression; viral transcription; rRNA processing

Disease: Diamond-blackfan Anemia 3