Rabbit anti-Human RPS15 Polyclonal Antibody | anti-RPS15 antibody

RPS15, NT (40S Ribosomal Protein S15, RIG Protein, RIG) (Azide free) (HRP)

Cat. Num. AAA6497744

• Gene Names: RPS15; RIG; S15
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: RPS15; Polyclonal Antibody; NT (40S Ribosomal Protein S15; RIG Protein; RIG) (Azide free) (HRP); anti-RPS15 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RPS15.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-RPS15 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human RPS15 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of RPS15 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). RPS15 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with RPS15 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of HepG2 cells using RPS15 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-RPS15 antibody

Antibodies; Ribosomal Proteins

References

1. Yu, Y., et.al., Protein Sci. 14(6), 1438-1446 (2005).

NCBI and Uniprot Product Information

• NCBI GI #: 4506687
• NCBI GeneID: 6209
• NCBI Accession #: NP_001009.1
• NCBI GenBank Nucleotide #: NM_001018.3
• UniProt Accession #: P62841; P11174; Q3KRA1; Q9UDC2; A5D8V9
• Molecular Weight: 17,040 Da
• NCBI Official Full Name: 40S ribosomal protein S15
• NCBI Official Synonym Full Names: ribosomal protein S15
• NCBI Official Symbol: RPS15
• NCBI Official Synonym Symbols: RIG; S15
• NCBI Protein Information: 40S ribosomal protein S15; insulinoma protein; homolog of rat insulinoma
• UniProt Protein Name: 40S ribosomal protein S15
• Protein Family: 30S ribosomal protein
• UniProt Gene Name: RPS15
• UniProt Synonym Gene Names: RIG
• UniProt Entry Name: RS15_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S19P family of ribosomal proteins. It is located in the cytoplasm. This gene has been found to be activated in various tumors, such as insulinomas, esophageal cancers, and colon cancers. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Uniprot Description

RPS15: a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S19P family of ribosomal proteins. It is located in the cytoplasm. This gene has been found to be activated in various tumors, such as insulinomas, esophageal cancers, and colon cancers. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Protein type: RNA-binding; Ribosomal

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleoplasm; focal adhesion; membrane; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; structural constituent of ribosome; DNA binding

Biological Process: ribosomal small subunit export from nucleus; SRP-dependent cotranslational protein targeting to membrane; viral reproduction; translation; translational termination; viral infectious cycle; osteoblast differentiation; ribosomal small subunit biogenesis and assembly; cellular protein metabolic process; translational elongation; translational initiation; mRNA catabolic process, nonsense-mediated decay; ribosomal small subunit assembly and maintenance; viral transcription; gene expression; rRNA processing