Rabbit RPL5 Polyclonal Antibody | anti-RPL5 antibody

RPL5 Polyclonal Antibody

Cat. Num. AAA127556

• Gene Names: RPL5; L5; DBA6; MSTP030; PPP1R135
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purification
• Synonyms: RPL5; Polyclonal Antibody; RPL5 Polyclonal Antibody; L5; DBA6; MSTP030; anti-RPL5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 297

• Applicable Applications for anti-RPL5 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200
• Species: Human
• Immunogen: Recombinant Protein
• Immunogen: Recombinant protein of human RPL5
• Calculated Molecular Weight: 34kDa
• Preparation and Storage: Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.; Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using RPL5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse liver using RPL5 antibody at dilution of 1:200 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse kidney using RPL5 antibody at dilution of 1:200 (40x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of MCF-7 cells using RPL5 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-RPL5 antibody

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L18P family of ribosomal proteins. It is located in the cytoplasm. The protein binds 5S rRNA to form a stable complex called the 5S ribonucleoprotein particle (RNP), which is necessary for the transport of nonribosome-associated cytoplasmic 5S rRNA to the nucleolus for assembly into ribosomes. The protein interacts specifically with the beta subunit of casein kinase II. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. This gene is co-transcribed with the small nucleolar RNA gene U21, which is located in its fifth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Product Categories/Family for anti-RPL5 antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 81175191
• NCBI GeneID: 6125
• UniProt Accession #: P46777; Q32LZ3; Q53HH6; Q9H3F4
• Molecular Weight: 297
• NCBI Official Full Name: 60S ribosomal protein L5
• NCBI Official Synonym Full Names: ribosomal protein L5
• NCBI Official Symbol: RPL5
• NCBI Official Synonym Symbols: L5; DBA6; MSTP030; PPP1R135
• NCBI Protein Information: 60S ribosomal protein L5; protein phosphatase 1, regulatory subunit 135
• UniProt Protein Name: 60S ribosomal protein L5
• Protein Family: 50S ribosomal protein
• UniProt Gene Name: RPL5
• UniProt Entry Name: RL5_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L18P family of ribosomal proteins. It is located in the cytoplasm. The protein binds 5S rRNA to form a stable complex called the 5S ribonucleoprotein particle (RNP), which is necessary for the transport of nonribosome-associated cytoplasmic 5S rRNA to the nucleolus for assembly into ribosomes. The protein interacts specifically with the beta subunit of casein kinase II. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. This gene is co-transcribed with the small nucleolar RNA gene U21, which is located in its fifth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Uniprot Description

RPL5: Required for rRNA maturation and formation of the 60S ribosomal subunits. This protein binds 5S RNA. Defects in RPL5 are the cause of Diamond-Blackfan anemia type 6 (DBA6). DBA6 is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies. Belongs to the ribosomal protein L18P family.

Protein type: Nucleolus; Ribosomal; Translation

Chromosomal Location of Human Ortholog: 1p22.1

Cellular Component: focal adhesion; membrane; cytoplasm; nucleolus; cytosol; ribonucleoprotein complex; nucleus

Molecular Function: protein binding; structural constituent of ribosome; RNA binding; 5S rRNA binding

Biological Process: SRP-dependent cotranslational protein targeting to membrane; viral reproduction; translation; translational termination; ribosomal large subunit biogenesis and assembly; viral infectious cycle; translational elongation; cellular protein metabolic process; translational initiation; mRNA catabolic process, nonsense-mediated decay; viral transcription; gene expression; rRNA processing

Disease: Diamond-blackfan Anemia 6