Rabbit anti-Human RPL31 Polyclonal Antibody | anti-RPL31 antibody

RPL31, ID (RPL31, 60S ribosomal protein L31)

Cat. Num. AAA6005365

• Gene Names: RPL31; L31
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: RPL31; Polyclonal Antibody; ID (RPL31; 60S ribosomal protein L31); Anti -RPL31; anti-RPL31 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-RPL31 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100; Flow Cytometry: 1:10-50
• Immunogen: RPL31 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Central region of human RPL31.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of RPL31 Antibody (Center) in Hela cell line lysates (35ug/lane).RPL31 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human testis tissue reacted with RPL31 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(RPL31 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-RPL31 antibody

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL31 is a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L31E family of ribosomal proteins. It is located in the cytoplasm.

Product Categories/Family for anti-RPL31 antibody

Antibodies; Abs to Ribosomal Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 47683009
• NCBI GeneID: 6160
• UniProt Accession #: P62899; P12947; Q53SQ5; Q6IRZ0; Q6LBJ6; B7Z4K2; D3DVJ4
• Molecular Weight: 14,463 Da
• NCBI Official Full Name: RPL31 protein
• NCBI Official Synonym Full Names: ribosomal protein L31
• NCBI Official Symbol: RPL31
• NCBI Official Synonym Symbols: L31
• NCBI Protein Information: 60S ribosomal protein L31
• UniProt Protein Name: 60S ribosomal protein L31
• UniProt Gene Name: RPL31
• UniProt Entry Name: RL31_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L31E family of ribosomal proteins. It is located in the cytoplasm. Higher levels of expression of this gene in familial adenomatous polyps compared to matched normal tissues have been observed. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

RPL31: a ribosomal protein of the L31E family. Located in the cytoplasm. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This protein is a component of the 60S subunit. Higher levels of expression in familial adenomatous polyps compared to matched normal tissues have been observed.

Protein type: Translation; Ribosomal

Chromosomal Location of Human Ortholog: 2q11.2

Cellular Component: focal adhesion; membrane; cytosol

Molecular Function: structural constituent of ribosome; RNA binding

Biological Process: SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; translational elongation; viral reproduction; translation; mRNA catabolic process, nonsense-mediated decay; translational initiation; viral transcription; gene expression; viral infectious cycle; translational termination