Rabbit RPL27A Polyclonal Antibody | anti-RPL27A antibody

RPL27A (V140) Polyclonal Antibody

Cat. Num. AAA3003353

• Gene Names: RPL27A; L27A
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: RPL27A; Polyclonal Antibody; RPL27A (V140) Polyclonal Antibody; 60S ribosomal protein L27a; anti-RPL27A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Ribosomal Protein L27A (V140) polyclonal antibody detects endogenous levels of Ribosomal Protein L27A protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 148

• Applicable Applications for anti-RPL27A antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 120-160 of Human Ribosomal Protein L27A.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of RPL27A (V140) pAb at 1:500 dilutionLane1:Hela whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (40ug) Lane3:The Brain tissue lysate of Mouse (40ug) Lane4:The Brain tissue lysate of Rat (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of RPL27A (V140) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.)

Related Product Information for anti-RPL27A antibody

Ribosomes, the organelles that catalyze protein synthesis, are composed of a small subunit (40S) and a large subunit (60S) that consist of over 80 distinct ribosomal proteins. Mammalian ribosomal proteins are encoded by multigene families that contain processed pseudogenes and one functional intron-containing gene within their coding regions. Ribosomal Protein L27, also known as RPL27, is a 136 amino acid protein belonging to the ribosomal protein L27e family exists as a component of the 60S subunit, possibly playing a role in protein translation. Like most ribosomal proteins, Ribosomal Protein L27 exists as multiple processed pseudogenes that are scattered throughout the genome. Considered a novel candidate housekeeping gene, the gene encoding Ribosomal Protein L27 maps to human chromosome 17, which comprises over 2.5% of the human genome and encodes over 1, 200 genes.

NCBI and Uniprot Product Information

• NCBI GI #: 4506625
• NCBI GeneID: 6157
• NCBI Accession #: NP_000981.1
• NCBI GenBank Nucleotide #: NP_000981.1
• UniProt Accession #: P46776
• Molecular Weight: ~ 17kDa
• NCBI Official Full Name: 60S ribosomal protein L27a
• NCBI Official Synonym Full Names: ribosomal protein L27a
• NCBI Official Symbol: RPL27A
• NCBI Official Synonym Symbols: L27A
• NCBI Protein Information: 60S ribosomal protein L27a
• UniProt Protein Name: 60S ribosomal protein L27a
• Protein Family: 60S ribosomal protein
• UniProt Gene Name: RPL27A
• UniProt Entry Name: RL27A_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L15P family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, multiple processed pseudogenes derived from this gene are dispersed through the genome. [provided by RefSeq, Jul 2008]

Uniprot Description

RPL27A: a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L15P family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, multiple processed pseudogenes derived from this gene are dispersed through the genome. [provided by RefSeq, Jul 2008]

Protein type: Ribosomal; Translation

Chromosomal Location of Human Ortholog: 11p15

Cellular Component: membrane; cytosol

Molecular Function: protein binding; structural constituent of ribosome; RNA binding

Biological Process: SRP-dependent cotranslational protein targeting to membrane; translational elongation; cellular protein metabolic process; translation; viral reproduction; mRNA catabolic process, nonsense-mediated decay; translational initiation; gene expression; viral transcription; translational termination; viral infectious cycle