Rabbit anti-Mouse Rpl13a Polyclonal Antibody | anti-Rpl13a antibody

Rpl13a, NT (Rpl13a, P198, Tstap198-7, 60S ribosomal protein L13a, Transplantation antigen P198, Tum-P198 antigen) (APC)

Cat. Num. AAA6343359

• Gene Names: Rpl13a; Tstap198-7; tum-antigen; 1810026N22Rik
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Rpl13a; Polyclonal Antibody; NT (Rpl13a; P198; Tstap198-7; 60S ribosomal protein L13a; Transplantation antigen P198; Tum-P198 antigen) (APC); Tum-P198 antigen; anti-Rpl13a antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-Rpl13a antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse Rpl13a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28-54 amino acids from the N-terminal region of mouse Rpl13a.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Mouse Rpl13a Antibody (N-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the Rpl13a antibody detected the Rpl13a protein (arrow).)

Related Product Information for anti-Rpl13a antibody

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L13P family of ribosomal proteins. It is located in the cytoplasm. Transcript variants utilizing alternative polyA signals have been observed. This gene is co-transcribed with the small nucleolar RNA genes U32, U33, U34, and U35, which are located in its second, fourth, fifth, and sixth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Product Categories/Family for anti-Rpl13a antibody

Antibodies; Ribosomal Proteins

References

Maggi, L.B. Jr., et al. Mol. Cell. Biol. 28(23):7050-7065(2008). Stryke, D., et al. Nucleic Acids Res. 31(1):278-281(2003). Mahy, N.L., et al. J. Cell Biol. 159(5):753-763(2002). Neidhardt, L., et al. Mech. Dev. 98 (1-2), 77-94 (2000): Gu, Z., et al. Mol. Cell. Biol. 20(1):233-241(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 31981945
• NCBI GeneID: 22121
• NCBI Accession #: NP_033464.2
• NCBI GenBank Nucleotide #: NM_009438.5
• UniProt Accession #: P19253
• Molecular Weight: 23,464 Da
• NCBI Official Full Name: 60S ribosomal protein L13a
• NCBI Official Synonym Full Names: ribosomal protein L13A
• NCBI Official Symbol: Rpl13a
• NCBI Official Synonym Symbols: Tstap198-7; tum-antigen; 1810026N22Rik
• NCBI Protein Information: 60S ribosomal protein L13a
• UniProt Protein Name: 60S ribosomal protein L13a
• Protein Family: 60S ribosomal protein
• UniProt Gene Name: Rpl13a
• UniProt Synonym Gene Names: P198; Tstap198-7

Uniprot Description

Associated with ribosomes but is not required for canonical ribosome function and has extra-ribosomal functions (). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma activation and subsequent phosphorylation dissociates from the ribosome and assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. In the GAIT complex interacts with m7G cap-bound eIF4G at or near the eIF3-binding site and blocks the recruitment of the 43S ribosomal complex.