Rabbit anti-Human RPL12 Polyclonal Antibody | anti-RPL12 antibody

RPL12, ID (RPL12, 60S ribosomal protein L12) (PE)

Cat. Num. AAA6343357

• Gene Names: RPL12; L12
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RPL12; Polyclonal Antibody; ID (RPL12; 60S ribosomal protein L12) (PE); 60S ribosomal protein L12; anti-RPL12 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-RPL12 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RPL12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-100 amino acids from the Central region of human RPL12.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(RPL12 Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the RPL12 antibody detected the RPL12 protein (arrow).)

Related Product Information for anti-RPL12 antibody

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L11P family of ribosomal proteins. It is located in the cytoplasm. The protein binds directly to the 26S rRNA. This gene is co-transcribed with the U65 snoRNA, which is located in its fourth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Product Categories/Family for anti-RPL12 antibody

Antibodies; Ribosomal Proteins

References

Letra, A., et al. Am. J. Med. Genet. A 152A (7), 1701-1710 (2010): Wheeler, H.E., et al. PLoS Genet. 5 (10), E1000685 (2009): Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007). Olsen, J.V., et al. Cell 127(3):635-648(2006). Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 4506597
• NCBI GeneID: 6136
• NCBI Accession #: NP_000967.1
• NCBI GenBank Nucleotide #: NM_000976.3
• UniProt Accession #: P30050; Q5VVV2; Q6PB27
• Molecular Weight: 17,819 Da
• NCBI Official Full Name: 60S ribosomal protein L12
• NCBI Official Synonym Full Names: ribosomal protein L12
• NCBI Official Symbol: RPL12
• NCBI Official Synonym Symbols: L12
• NCBI Protein Information: 60S ribosomal protein L12
• UniProt Protein Name: 60S ribosomal protein L12
• Protein Family: 50S ribosomal protein
• UniProt Gene Name: RPL12
• UniProt Entry Name: RL12_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L11P family of ribosomal proteins. It is located in the cytoplasm. The protein binds directly to the 26S rRNA. This gene is co-transcribed with the U65 snoRNA, which is located in its fourth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Uniprot Description

RPL12: a ribosomal protein of the L11P family. Located in the cytoplasm. Binds directly to the 26S rRNA. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This protein is a component of the 60S subunit.

Protein type: Translation; Ribosomal

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: focal adhesion; membrane; cytosol

Molecular Function: protein binding; structural constituent of ribosome

Biological Process: SRP-dependent cotranslational protein targeting to membrane; translational elongation; cellular protein metabolic process; translation; viral reproduction; translational initiation; mRNA catabolic process, nonsense-mediated decay; viral transcription; gene expression; viral infectious cycle; translational termination