Rabbit RPL11 Polyclonal Antibody | anti-RPL11 antibody

RPL11 Polyclonal Antibody

Cat. Num. AAA3011793

• Gene Names: RPL11; L11; uL5; DBA7; GIG34
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: RPL11; Polyclonal Antibody; RPL11 Polyclonal Antibody; 60S ribosomal protein L11; CLL-associated antigen KW-12; anti-RPL11 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RPL11 polyclonal antibody detects endogenous levels of RPL11 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 178

• Applicable Applications for anti-RPL11 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: Recombinant full length Human RPL11.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using RPL11 antibody.)

Related Product Information for anti-RPL11 antibody

The genes encoding for mammalian ribosomal proteins comprise multigene families that consist predominantly of multiple processed pseudogenes and one functional intro-containing gene within their coding regions. The rpS6 gene gives rise to Ribosomal protein S6 (also designated RPS6) and Ribosomal protein L28. Sequence comparison has identified RPS6 as the equivalent of the ribosomal protein S10 from Saccharomyces cerevisiae. The sequence comparison of ribosomal proteins from evolutionarily distant eukaryotes, such as yeast and human, indicates that the structure and the function are highly conserved.

NCBI and Uniprot Product Information

• NCBI GI #: 15431290
• NCBI GeneID: 6135
• NCBI Accession #: NP_000966.2
• NCBI GenBank Nucleotide #: NP_000966.2
• UniProt Accession #: P62913
• Molecular Weight: ~ 20kDa
• NCBI Official Full Name: 60S ribosomal protein L11 isoform 1
• NCBI Official Synonym Full Names: ribosomal protein L11
• NCBI Official Symbol: RPL11
• NCBI Official Synonym Symbols: L11; uL5; DBA7; GIG34
• NCBI Protein Information: 60S ribosomal protein L11
• UniProt Protein Name: 60S ribosomal protein L11
• Protein Family: 50S ribosomal protein
• UniProt Gene Name: RPL11
• UniProt Entry Name: RL11_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L5P family of ribosomal proteins. It is located in the cytoplasm. The protein probably associates with the 5S rRNA. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Dec 2010]

Uniprot Description

RPL11: Binds to 5S ribosomal RNA. Required for rRNA maturation and formation of the 60S ribosomal subunits. Promotes nucleolar location of PML. Defects in RPL11 are the cause of Diamond-Blackfan anemia type 7 (DBA7). DBA7 is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies. Belongs to the ribosomal protein L5P family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus; Motility/polarity/chemotaxis; Ribosomal; Translation

Chromosomal Location of Human Ortholog: 1p36.1-p35

Cellular Component: membrane; cytoplasm; nucleolus; cytosol

Molecular Function: rRNA binding; protein binding; structural constituent of ribosome; RNA binding

Biological Process: SRP-dependent cotranslational protein targeting to membrane; viral reproduction; translation; ribosomal large subunit biogenesis and assembly; translational termination; viral infectious cycle; cellular protein metabolic process; translational elongation; translational initiation; mRNA catabolic process, nonsense-mediated decay; viral transcription; gene expression; rRNA processing; protein targeting

Disease: Diamond-blackfan Anemia 7