Western Blot (WB)
(Western blot analysis of RPC8 Antibody (N-term) in mouse testis tissue lysates (35ug/lane). RPC8 (arrow) was detected using the purified Pab.)
Rabbit anti-Human, Mouse RPC8 Polyclonal Antibody | anti-RPC31 antibody
RPC8, NT (POLR3H, KIAA1665, RPC8, DNA-directed RNA polymerase III subunit RPC8, DNA-directed RNA polymerase III subunit H, RNA polymerase III subunit 22.9kD subunit)
Gene Names
RPC31; ACP2; RPC8
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
RPC8; Polyclonal Antibody; NT (POLR3H; KIAA1665; DNA-directed RNA polymerase III subunit RPC8; DNA-directed RNA polymerase III subunit H; RNA polymerase III subunit 22.9kD subunit); Anti -RPC8; anti-RPC31 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Human, mouse
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-RPC31 antibody
ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Western Blot, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Flow Cytometry: 1:10-50
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Flow Cytometry: 1:10-50
Immunogen
RPC8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acid 10-40 from the N-terminal region of human RPC8.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of RPC8 Antibody (N-term) in mouse testis tissue lysates (35ug/lane). RPC8 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of RPC8 Antibody (N-term) in mouse testis tissue lysates (35ug/lane). RPC8 (arrow) was detected using the purified Pab.)
Flow Cytometry (FC/FACS)
(RPC8 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(RPC8 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-RPC31 antibody
RPC8 is DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. It is plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. It is acting as nuclear and cytosolic DNA sensor involved in innate immune response. It can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF-Kappa-B through the RIG-I pathway.
Product Categories/Family for anti-RPC31 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27,724 Da
NCBI Official Full Name
Rpc31p
NCBI Official Symbol
RPC31
NCBI Official Synonym Symbols
ACP2; RPC8
NCBI Protein Information
Rpc31p
UniProt Protein Name
DNA-directed RNA polymerase III subunit RPC7
Protein Family
UniProt Gene Name
RPC31
UniProt Synonym Gene Names
ACP2; RPC8; RNA polymerase III subunit C7; C31
UniProt Entry Name
RPC7_YEAST
Uniprot Description
Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. C31 is involved in the formation of the initiation complex.
Subunit structure: Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits.
Subcellular location: Nucleus.
Miscellaneous: The acidic domain is essential for its function.
Sequence similarities: Belongs to the eukaryotic RPC7 RNA polymerase subunit family.
Similar Products
Product Notes
The RPC31 rpc31 (Catalog #AAA645223) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RPC8, NT (POLR3H, KIAA1665, RPC8, DNA-directed RNA polymerase III subunit RPC8, DNA-directed RNA polymerase III subunit H, RNA polymerase III subunit 22.9kD subunit) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RPC8 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the RPC31 rpc31 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPC8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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