Rabbit RPC3 Polyclonal Antibody | anti-POLR3C antibody

RPC3 Antibody

Cat. Num. AAA9630318

• Gene Names: POLR3C; RPC3; RPC62
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: RPC3; Polyclonal Antibody; RPC3 Antibody; DNA directed III 62 kDa polypeptide; DNA-directed RNA polymerase III subunit C; DNA-directed RNA polymerase III subunit rpc3; POLR3C; Polymerase (RNA) III (DNA directed) polypeptide C (62kD); RNA polymerase C subunit 3; RNA polymerase III 62 kDa subunit; RNA polymerase III C62 subunit; RNA polymerase III subunit C3; RPC3_HUMAN; RPC62; anti-POLR3C antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: RPC3 Antibody detects endogenous levels of RPC3.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-POLR3C antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human RPC3.
• Conjugation: Unconjugated
• Similarity: Belongs to the eukaryotic RPC3/POLR3C RNA polymerase subunit family.
• Subunit Structure: Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits (By similarity). RPC3/POLR3C, RPC6/POLR3F and RPC7/POLR3G form a Pol III subcomplex. Directly interacts with POLR3G and POLR3GL. Directly interacts with POLR3F/RPC39. Interacts with GTF3C4.
• Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. Preferentially binds single-stranded DNA (ssDNA) in a sequence-independent manner.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-POLR3C antibody

Nucleus; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 21359969
• NCBI GeneID: 10623
• NCBI Accession #: NP_006459.3
• NCBI GenBank Nucleotide #: NM_006468.6
• UniProt Accession #: Q9BUI4; O15317; Q9Y3R6
• Molecular Weight: 60,612 Da
• NCBI Official Full Name: DNA-directed RNA polymerase III subunit RPC3
• NCBI Official Synonym Full Names: polymerase (RNA) III (DNA directed) polypeptide C (62kD)
• NCBI Official Symbol: POLR3C
• NCBI Official Synonym Symbols: RPC3; RPC62
• NCBI Protein Information: DNA-directed RNA polymerase III subunit RPC3; RNA polymerase III subunit C3; RNA polymerase III 62 kDa subunit; DNA-directed III 62 kDa polypeptide; DNA-directed RNA polymerase III subunit C
• UniProt Protein Name: DNA-directed RNA polymerase III subunit RPC3
• UniProt Gene Name: POLR3C
• UniProt Synonym Gene Names: RNA polymerase III subunit C3; RPC62
• UniProt Entry Name: RPC3_HUMAN

Uniprot Description

POLR3C: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the subcomplex RNA Pol III binding to the TFIIIB- DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. Belongs to the eukaryotic RPC3/POLR3C RNA polymerase subunit family.

Protein type: Transferase; Transcription initiation complex; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; EC 2.7.7.6

Chromosomal Location of Human Ortholog: 1q21.1

Cellular Component: nucleoplasm; DNA-directed RNA polymerase III complex; nucleus; cytosol

Molecular Function: DNA binding; DNA-directed RNA polymerase activity

Biological Process: transcription from RNA polymerase III promoter; termination of RNA polymerase III transcription; regulation of transcription from RNA polymerase III promoter; positive regulation of innate immune response; positive regulation of interferon type I production; positive regulation of interferon-beta production; innate immune response; gene expression; defense response to virus; RNA elongation from RNA polymerase III promoter