Western Blot (WB)
(Roundabout 4 Antibody (N-term) western blot analysis in 293,K562,HL-60 cell line lysates (35ug/lane).This demonstrates the Roundabout4 antibody detected the Roundabout4 protein (arrow).)
Rabbit anti-Human Roundabout 4 Polyclonal Antibody | anti-ROBO4 antibody
Roundabout 4, NT (ROBO4, Roundabout homolog 4, Magic roundabout)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500Immunohistochemistry 1:50-100
Flow Cytometry: 1:10-50
Western Blot (WB)
(Roundabout 4 Antibody (N-term) western blot analysis in 293,K562,HL-60 cell line lysates (35ug/lane).This demonstrates the Roundabout4 antibody detected the Roundabout4 protein (arrow).)
Western Blot (WB)
(Roundabout 4 Antibody (N-term) western blot analysis in 293,K562,HL-60 cell line lysates (35ug/lane).This demonstrates the Roundabout4 antibody detected the Roundabout4 protein (arrow).)
Immunohistochemistry (IHC)
(Roundabout 4 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Roundabout 4 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Roundabout 4 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Roundabout 4 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(Roundabout 4 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Roundabout 4 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
Uniprot Description
ROBO4: Receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. May mediate the inhibition of primary endothelial cell migration by Slit proteins. Belongs to the immunoglobulin superfamily. ROBO family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 11q24.2
Cellular Component: integral to membrane; external side of plasma membrane
Molecular Function: receptor activity
Biological Process: angiogenesis; cell differentiation; regulation of cell migration; negative regulation of cell migration
Research Articles on ROBO4
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Product Notes
The ROBO4 robo4 (Catalog #AAA6000757) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Roundabout 4, NT (ROBO4, Roundabout homolog 4, Magic roundabout) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Roundabout 4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry Dilution: ELISA: 1:1,000 Western Blot: 1:100-500Immunohistochemistry 1:50-100 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the ROBO4 robo4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Roundabout 4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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