Rabbit ROS Polyclonal Antibody | anti-ROS antibody

ROS (Phospho-Y2114) Polyclonal Antibody

Cat. Num. AAA3007874

• Gene Names: ROS1; ROS; MCF3; c-ros-1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ROS; Polyclonal Antibody; ROS (Phospho-Y2114) Polyclonal Antibody; Proto-oncogene tyrosine-protein kinase ROS; Proto-oncogene c-Ros; Proto-oncogene c-Ros-1; Receptor tyrosine kinase c-ros oncogene 1; c-Ros receptor tyrosine kinase; ROS1; MCF3; anti-ROS antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: p-ROS (Y2114) polyclonal antibody detects endogenous levels of human ROS only when phosphorylated at Tyr2114.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 2347

• Applicable Applications for anti-ROS antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic phosphopeptide derived from human ROS around the phosphorylation site of Tyrosine 2114.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of p-ROS (Y2114) pAb at 1:500 dilutionLane1:C6 whole cell lysate (40ug) Lane2:Hela whole cell lysate (40ug) Lane3:The Brain tissue lysate of Mouse (40ug) Lane4:The Brain tissue lysate of Rat (40ug) Lane5:HEK293T whole cell lysate (40ug) Lane6:A549 whole cell lysate (40ug))

Related Product Information for anti-ROS antibody

LTK, ALK and Ros have been identified as receptor tyrosine kinases having sequence similarity to the insulin receptor subfamily of kinases. LTK, leukocyte tyrosine kinase, is expressed in murine B-lymphocyte precursors and has also been found in forebrain neurons. ALK, anaplastic lymphoma kinase, is normally highly expressed specifically in the nervous system. A truncated form containing the catalytic domian of ALK is expressed as the result of a translocation occuring in many non-Hodgkins lymphomas. The c-ros gene was originally identified in mutant form as an oncogene. Ros is normally expressed in a small number of epithelial cell types and may play a role in epithelial development.

NCBI and Uniprot Product Information

• NCBI GI #: 19924165
• NCBI GeneID: 6098
• NCBI Accession #: NP_002935.2
• NCBI GenBank Nucleotide #: NP_002935.2
• UniProt Accession #: P08922
• Molecular Weight: ~ 55-80kDa
• NCBI Official Full Name: proto-oncogene tyrosine-protein kinase ROS isoform 1
• NCBI Official Synonym Full Names: ROS proto-oncogene 1, receptor tyrosine kinase
• NCBI Official Symbol: ROS1
• NCBI Official Synonym Symbols: ROS; MCF3; c-ros-1
• NCBI Protein Information: proto-oncogene tyrosine-protein kinase ROS
• UniProt Protein Name: Proto-oncogene tyrosine-protein kinase ROS
• Protein Family: Rosmarinate synthase
• UniProt Gene Name: ROS1
• UniProt Synonym Gene Names: MCF3; ROS
• UniProt Entry Name: ROS1_HUMAN

NCBI Description

This proto-oncogene, highly-expressed in a variety of tumor cell lines, belongs to the sevenless subfamily of tyrosine kinase insulin receptor genes. The protein encoded by this gene is a type I integral membrane protein with tyrosine kinase activity. The protein may function as a growth or differentiation factor receptor. [provided by RefSeq, Jul 2008]

Uniprot Description

ROS: a proto-oncogenic receptor tyrosine kinase whose expression is tightly restricted during development. Normally expressed in adult murine and human epithelial cells of the epididymis. Transgenic mice lacking the c-ros gene are infertile. Ectopic expression of c-Ros has been reported in meningiomas and astrocytomas. Is up-regulated in human glioma: 30% of malignant glioma tumors are ROS positive. An oncogenic fusion protein between PIST (aka FIG) and ROS, resulting from an intra-chromosomal homozygous deletion of 240 kilobases on 6q21, is found in glioblastoma multiform. PIST (aka FIG) is a peripheral membrane protein associated with the Golgi apparatus. Unlike other fusion RTK oncogenes, the mechanism of activation of PIST-ROS does not appear to be dimerization: the PIST-ROS fusion protein appears to be monomeric in vivo. Rather, activation of the fused ROS kinase appears to depend upon translocation to the golgi apparatus: deletion of 2nd coiled-coil region of PIST, crucial for Golgi localization, appears to eliminate the transformation capacity of PIST-ROS. c-ROS may also be activated epigenetically, suggesting caution when using 5-aza-dC for treating glioma.

Protein type: Kinase, protein; Protein kinase, tyrosine (receptor); Oncoprotein; EC 2.7.10.1; Protein kinase, TK; Membrane protein, integral; TK group; Sev family

Chromosomal Location of Human Ortholog: 6q22

Cellular Component: membrane; integral to membrane; plasma membrane

Molecular Function: protein binding; protein-tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; protein phosphatase binding; ATP binding

Biological Process: cell proliferation; peptidyl-tyrosine phosphorylation; spermatogenesis; cell growth; cell differentiation; columnar/cuboidal epithelial cell development; protein amino acid phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of TOR signaling pathway