Rabbit anti-Human RORC Polyclonal Antibody | anti-RORC antibody

RORC, ID (RORC, NR1F3, RORG, RZRG, Nuclear receptor ROR-gamma, Nuclear receptor RZR-gamma, Nuclear receptor subfamily 1 group F member 3, Retinoid-related orphan receptor-gamma) (Azide free) (HRP)

Cat. Num. AAA6343131

• Gene Names: RORC; TOR; RORG; RZRG; NR1F3; RZR-GAMMA
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RORC; Polyclonal Antibody; ID (RORC; NR1F3; RORG; RZRG; Nuclear receptor ROR-gamma; Nuclear receptor RZR-gamma; Nuclear receptor subfamily 1 group F member 3; Retinoid-related orphan receptor-gamma) (Azide free) (HRP); Retinoid-related orphan receptor-gamma; anti-RORC antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-RORC antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RORC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-216 amino acids from the Central region of human RORC.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RORC Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the RORC antibody detected the RORC protein (arrow).)

Related Product Information for anti-RORC antibody

The protein encoded by this gene is a DNA-binding transcription factor and is a member of the NR1 subfamily of nuclear hormone receptors. The specific functions of this protein are not known; however, studies of a similar gene in mice have shown that this gene may be essential for lymphoid organogenesis and may play an important regulatory role in thymopoiesis. In addition, studies in mice suggest that the protein encoded by this gene may inhibit the expression of Fas ligand and IL2. Two transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-RORC antibody

Antibodies; Receptors

References

Wang, Y., et al. Biochim. Biophys. Acta 1801(8):917-923(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Burgler, S., et al. J. Immunol. 184(11):6161-6169(2010). Jin, L., et al. Mol. Endocrinol. 24(5):923-929(2010). Wang, W.J., et al. J. Reprod. Immunol. 84(2):164-170(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 48255918
• NCBI GeneID: 6097
• NCBI Accession #: NP_001001523.1; NP_005051.2
• NCBI GenBank Nucleotide #: NM_001001523.1
• UniProt Accession #: P51449; Q5SZR9; Q8N5V7; Q8NCY8
• Molecular Weight: 58,195 Da
• NCBI Official Full Name: nuclear receptor ROR-gamma isoform b
• NCBI Official Synonym Full Names: RAR-related orphan receptor C
• NCBI Official Symbol: RORC
• NCBI Official Synonym Symbols: TOR; RORG; RZRG; NR1F3; RZR-GAMMA
• NCBI Protein Information: nuclear receptor ROR-gamma; nuclear receptor RZR-gamma; retinoic acid-binding receptor gamma; retinoid-related orphan receptor gamma; RAR-related orphan receptor C, isoform a; RAR-related orphan nuclear receptor variant 2; nuclear receptor subfamily 1 gro
• UniProt Protein Name: Nuclear receptor ROR-gamma
• Protein Family: Nuclear receptor
• UniProt Gene Name: RORC
• UniProt Synonym Gene Names: NR1F3; RORG; RZRG
• UniProt Entry Name: RORG_HUMAN

NCBI Description

The protein encoded by this gene is a DNA-binding transcription factor and is a member of the NR1 subfamily of nuclear hormone receptors. The specific functions of this protein are not known; however, studies of a similar gene in mice have shown that this gene may be essential for lymphoid organogenesis and may play an important regulatory role in thymopoiesis. In addition, studies in mice suggest that the protein encoded by this gene may inhibit the expression of Fas ligand and IL2. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

RORC: Possible nuclear receptor for hydroxycholesterols, the binding of which strongly promotes coactivators recruitment. Essential for thymopoiesis and the development of several secondary lymphoid tissues, including lymph nodes. Involved in lineage specification of uncommitted CD4(+) T-helper cells into Th17 cells. Regulate the expression of several components of the circadian clock. Belongs to the nuclear hormone receptor family. NR1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Nuclear receptor; Transcription factor

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; ligand-dependent nuclear receptor activity; DNA binding; zinc ion binding; sequence-specific DNA binding; steroid hormone receptor activity; oxysterol binding; transcription factor activity

Biological Process: Peyer's patch development; transcription initiation from RNA polymerase II promoter; intracellular receptor-mediated signaling pathway; T-helper cell differentiation; regulation of fat cell differentiation; positive regulation of transcription, DNA-dependent; T cell differentiation in the thymus; positive regulation of circadian rhythm; lymph node development; protein amino acid phosphorylation; regulation of gamma-delta T cell differentiation; xenobiotic metabolic process; regulation of steroid metabolic process; steroid hormone mediated signaling; gene expression; circadian regulation of gene expression