Rabbit RON Polyclonal Antibody | anti-RON antibody

RON Antibody

Cat. Num. AAA9613641

• Gene Names: MST1R; RON; PTK8; CD136; CDw136
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Synonyms: RON; Polyclonal Antibody; RON Antibody; c met related tyrosine kinase; CD136; CD136 antigen; CDw136; Macrophage stimulating 1 receptor (c met related tyrosine kinase); Macrophage stimulating 1 receptor; Macrophage stimulating protein receptor alpha chain; MACROPHAGE STIMULATING PROTEIN RECEPTOR; Macrophage stimulating protein receptor beta chain; Macrophage-Stimulating 1 Receptor (MST1R); Macrophage-stimulating protein receptor beta chain; MSP receptor; Mst1r; MST1R variant RON30; MST1R variant RON62; NPCA3; p185 RON; p185-Ron; Protein-tyrosine kinase 8; PTK 8; ptk8; PTK8 protein tyrosine kinase 8; Recepteur d'origine nantais (RON); RON protein tyrosine kinase; RON variant E2E3; RON_HUMAN; Soluble RON variant 1; Soluble RON variant 2; Soluble RON variant 3; Soluble RON variant 4; Stem cell derived tyrosine kinase; anti-RON antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: RON Antibody detects endogenous levels of total RON.; Tissue Specificity: Expressed in colon, skin, lung and bone marrow.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-RON antibody: Peptide ELISA (EIA)
• Application Notes: Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human RON.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Proteolytic processing yields the two subunits. Autophosphorylated in response to ligand binding on Tyr-1238 and Tyr-1239 in the kinase domain leading to further phosphorylation of Tyr-1353 and Tyr-1360 in the C-terminal multifunctional docking site.Ubiquitinated. Ubiquitination by CBL regulates the receptor stability and activity through proteasomal degradation.
• Subunit Structure: Heterodimer of an alpha chain and a beta chain which are disulfide linked. Binds PLXNB1. Associates with and is negatively regulated by HYAL2. Interacts when phosphorylated with downstream effectors including PIK3R1, PCLG1, GRB2 and GAB1. Interacts with integrin beta1/ITGB1 in a ligand-independent fashion.
• Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family.
• Subcellular Location: Membrane>Single-pass type I membrane protein.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Related Product Information for anti-RON antibody

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to MST1 ligand. Regulates many physiological processes including cell survival, migration and differentiation. Ligand binding at the cell surface induces autophosphorylation of RON on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1 or the adapter GAB1. Recruitment of these downstream effectors by RON leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. RON signaling activates the wound healing response by promoting epithelial cell migration, proliferation as well as survival at the wound site. Plays also a role in the innate immune response by regulating the migration and phagocytic activity of macrophages. Alternatively, RON can also promote signals such as cell migration and proliferation in response to growth factors other than MST1 ligand.

NCBI and Uniprot Product Information

• NCBI GI #: 349732251
• NCBI GeneID: 4486
• NCBI Accession #: NP_001231866.1
• NCBI GenBank Nucleotide #: NM_001244937.1
• UniProt Accession #: Q04912; B5A944; B5A945; B5A946; B5A947
• Molecular Weight: 152,271 Da
• NCBI Official Full Name: macrophage-stimulating protein receptor isoform 2
• NCBI Official Synonym Full Names: macrophage stimulating 1 receptor (c-met-related tyrosine kinase)
• NCBI Official Symbol: MST1R
• NCBI Official Synonym Symbols: RON; PTK8; CD136; CDw136
• NCBI Protein Information: macrophage-stimulating protein receptor; p185-Ron; MSP receptor; RON variant 21; RON variant E2E3; MST1R variant RON30; MST1R variant RON62; soluble RON variant 1; soluble RON variant 2; soluble RON variant 3; soluble RON variant 4; c-met-related tyrosine
• UniProt Protein Name: Macrophage-stimulating protein receptor
• Protein Family: Rondonin
• UniProt Gene Name: MST1R
• UniProt Synonym Gene Names: PTK8; RON; MSP receptor
• UniProt Entry Name: RON_HUMAN

NCBI Description

This gene encodes a cell surface receptor for macrophage-stimulating protein (MSP) with tyrosine kinase activity. The mature form of this protein is a heterodimer of disulfide-linked alpha and beta subunits, generated by proteolytic cleavage of a single-chain precursor. The beta subunit undergoes tyrosine phosphorylation upon stimulation by MSP. This protein is expressed on the ciliated epithelia of the mucociliary transport apparatus of the lung, and together with MSP, thought to be involved in host defense. Alternatively spliced transcript variants encoding different isoforms with different structural and biochemical properties have been described (PMID:8816464). [provided by RefSeq, Oct 2011]

Uniprot Description

Ron: a receptor tyrosine kinase of the Met family. Receptor for macrophage stimulating protein. Can be desensitized by c-Cbl and its binding partner Grb2. Overexpression of wild-type RON causes the formation of lung tumors. Two alternatively spliced isoforms have been described.

Protein type: Protein kinase, TK; EC 2.7.10.1; Membrane protein, integral; Kinase, protein; Protein kinase, tyrosine (receptor); TK group; Met family

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: cell surface; integral to plasma membrane; stress fiber

Molecular Function: macrophage colony stimulating factor receptor activity; protein binding; enzyme binding; ATP binding

Biological Process: positive regulation of MAP kinase activity; positive regulation of protein kinase B signaling cascade; peptidyl-tyrosine phosphorylation; multicellular organismal development; response to virus; single fertilization; positive regulation of cell proliferation; innate immune response; defense response; signal transduction; cell motility; transmembrane receptor protein tyrosine kinase signaling pathway