Rabbit anti-Human, Rat ROCK2 Polyclonal Antibody | anti-ROCK2 antibody

ROCK2 Antibody

Cat. Num. AAA1492984

• Gene Names: ROCK2; ROCK-II
• Reactivity: Human, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antigen Affinity Purified
• Synonyms: ROCK2; Polyclonal Antibody; ROCK2 Antibody; Rho kinase 2; Rho-associated; coiled-coil-containing protein kinase 2; coiled-coil-containing protein kinase II; ROCK-II; p164 ROCK-2; anti-ROCK2 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Sequence Length: 1388

• Applicable Applications for anti-ROCK2 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Recommended dilution: WB:1:200-1000; IHC:1:20-1:200
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Conjugate: Non-conjugated
• Immunogen: Recombinant human Rho-associated protein kinase 2 protein (1109-1388aa)
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-1851 / sc-1852 / sc-5561
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes: ROCK2 antibody at 2ug/ml+rat brain tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 161kDaObserved band size: 161kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human small intestine using MBS1492984 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human kidney tissue using MBS1492984 at dilution 1:100)

Immunofluorescence (IF)

(Immunofluorescent analysis of Hepg2 cells using MBS1492984 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-ROCK2 antibody

Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca2+ sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.

NCBI and Uniprot Product Information

• NCBI GI #: 41872583
• NCBI GeneID: 9475
• NCBI Accession #: NP_004841.2
• NCBI GenBank Nucleotide #: NM_004850.3
• UniProt Accession #: O75116; Q53QZ0; Q53SJ7; Q9UQN5
• Molecular Weight: 160,900 Da
• NCBI Official Full Name: rho-associated protein kinase 2
• NCBI Official Synonym Full Names: Rho associated coiled-coil containing protein kinase 2
• NCBI Official Symbol: ROCK2
• NCBI Official Synonym Symbols: ROCK-II
• NCBI Protein Information: rho-associated protein kinase 2
• UniProt Protein Name: Rho-associated protein kinase 2
• Protein Family: Rho-associated protein kinase
• UniProt Gene Name: ROCK2
• UniProt Synonym Gene Names: KIAA0619; ROCK-II
• UniProt Entry Name: ROCK2_HUMAN

NCBI Description

The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq, Jul 2008]

Uniprot Description

ROCK2: Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Homodimer. Interacts with IRS1, RHOB and RHOC. Interacts with RHOA (activated by GTP), PPP1R12A, CHORDC1, SORL1, EP300 and BRCA2. Interacts with NPM1 and this interaction enhances its activity. Interacts with RAF1. Activated by RHOA binding. Inhibited by Y-27632. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

Protein type: EC 2.7.11.1; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; AGC group; DMPK family; ROCK subfamily

Chromosomal Location of Human Ortholog: 2p24

Cellular Component: centrosome; cytosol; nucleus; plasma membrane

Molecular Function: ATP binding; metal ion binding; protein binding; protein serine/threonine kinase activity; Rho GTPase binding; structural molecule activity

Biological Process: axon guidance; centrosome duplication; cortical actin cytoskeleton organization and biogenesis; cytokinesis; ephrin receptor signaling pathway; I-kappaB kinase/NF-kappaB cascade; negative regulation of angiogenesis; positive regulation of protein amino acid phosphorylation; protein amino acid phosphorylation; regulation of actin cytoskeleton organization and biogenesis; regulation of cell adhesion; regulation of circadian rhythm; regulation of focal adhesion formation; regulation of keratinocyte differentiation; regulation of stress fiber formation; Rho protein signal transduction; rhythmic process; small GTPase mediated signal transduction; smooth muscle contraction; vascular endothelial growth factor receptor signaling pathway