Goat ROBO3 Polyclonal Antibody | anti-ROBO3 antibody
Goat anti-ROBO3 (internal) Antibody
Western Blot: Preliminary experiments gave an approx 25kDa band in Human Brain lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 148kDa according to NP_071765.2. The 25kDa band was successfully blocked by incubation with the immunizing peptide.
Flow cytometry: This antibody was successfully used on rheumatoid arthritis synovial fibroblasts (RASF) as described in Denk et al, Arthritis Res Ther.2010;12(2): R45.; PMID: 20298552.
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the Roundabout (ROBO) gene family that controls neurite outgrowth, growth cone guidance, and axon fasciculation. ROBO proteins are a subfamily of the immunoglobulin transmembrane receptor superfamily. SLIT proteins 1-3, a family of secreted chemorepellants, are ligands for ROBO proteins and SLIT/ROBO interactions regulate myogenesis, leukocyte migration, kidney morphogenesis, angiogenesis, and vasculogenesis in addition to neurogenesis. This gene, ROBO3, has a putative extracellular domain with five immunoglobulin (Ig)-like loops and three fibronectin (Fn) type III motifs, a transmembrane segment, and a cytoplasmic tail with three conserved signaling motifs: CC0, CC2, and CC3 (CC for conserved cytoplasmic). Unlike other ROBO family members, ROBO3 lacks motif CC1. The ROBO3 gene regulates axonal navigation at the ventral midline of the neural tube. In mouse, loss of Robo3 results in a complete failure of commissural axons to cross the midline throughout the spinal cord and the hindbrain. Mutations ROBO3 result in horizontal gaze palsy with progressive scoliosis (HGPPS); an autosomal recessive disorder characterized by congenital absence of horizontal gaze, progressive scoliosis, and failure of the corticospinal and somatosensory axon tracts to cross the midline in the medulla. Alternative transcript variants have been described but have not been experimentally validated. [provided by RefSeq, Dec 2009]
Uniprot Description
ROBO3: Thought to be involved during neural development in axonal navigation at the ventral midline of the neural tube. In spinal chord development plays a role in guiding commissural axons probably by preventing premature sensitivity to Slit proteins thus inhibiting Slit signaling through ROBO1. Required for hindbrain axon midline crossing. Defects in ROBO3 are a cause of familial horizontal gaze palsy with progressive scoliosis (HGPPS). Patients show a medulla where motor and sensory projections appear uncrossed. Belongs to the immunoglobulin superfamily. ROBO family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 11q24.2
Cellular Component: axon; integral to membrane
Biological Process: axon guidance; axon midline choice point recognition
Disease: Gaze Palsy, Familial Horizontal, With Progressive Scoliosis
Research Articles on ROBO3
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Product Notes
The ROBO3 robo3 (Catalog #AAA422569) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-ROBO3 (internal) Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's ROBO3 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Flow Cytometry (FC/FACS). Peptide ELISA: Antibody detection limit dilution 1: 64000. Western Blot: Preliminary experiments gave an approx 25kDa band in Human Brain lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 148kDa according to NP_071765.2. The 25kDa band was successfully blocked by incubation with the immunizing peptide. Flow cytometry: This antibody was successfully used on rheumatoid arthritis synovial fibroblasts (RASF) as described in Denk et al, Arthritis Res Ther.2010;12(2): R45.; PMID: 20298552. Researchers should empirically determine the suitability of the ROBO3 robo3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: RKDGARLKEE EGR. It is sometimes possible for the material contained within the vial of "ROBO3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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