Goat RNF34/RFI Polyclonal Antibody | anti-RNF34 antibody

Goat anti-RNF34/RFI (internal) Antibody

Cat. Num. AAA420625

• Gene Names: RNF34; RFI; RIF; RIFF; hRFI; CARP1; CARP-1
• Reactivity: Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
• Applications: ELISA
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: RNF34/RFI; Polyclonal Antibody; Goat anti-RNF34/RFI (internal) Antibody; Goat Anti-RNF34 / RFI (N Terminus) Antibody; RNF34; ring finger protein 34; FLJ21786; RFI; RIF; RIFF; FYVE-RING finger protein MOMO; RING finger protein RIFF; hRFI; RNF34 antibody; ring finger protein 34 antibody; FLJ21786 antibody; RFI antibody; RIF antibody; RIFF antibody; FYVE-RING finger protein MOMO antibody; RING finger protein RIFF antibody; hRFI antibody; RNF34 / RFI (internal); anti-RNF34 antibody

• Host: Goat
• Reactivity: Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: RLYKENEENQKSY
• Sequence Length: 373

• Applicable Applications for anti-RNF34 antibody: Peptide ELISA (EIA)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 64000.; Western Blot: Preliminary experiments gave an approx 35kDa band in Human Lymph Node, Ovary and Testis lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 41.7kDa according to NP_919247.1. The 35kDa band was successfully blocked by incubation with the immunizing peptide.
• Immunogen: Peptide with sequence C-RLYKENEENQKSY, from the internal region of the protein sequence according to NP_919247.1; NP_079402.2.
• Epitope: Internal region
• Note: This antibody is expected to recognize both reported isoforms (NP_919247.1; NP_079402.2).
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 37595539
• NCBI GeneID: 80196
• NCBI Accession #: NP_919247.1; NP_079402.2
• NCBI GenBank Nucleotide #: NM_194271.2
• Molecular Weight: 41,797 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase RNF34 isoform 1
• NCBI Official Synonym Full Names: ring finger protein 34
• NCBI Official Symbol: RNF34
• NCBI Official Synonym Symbols: RFI; RIF; RIFF; hRFI; CARP1; CARP-1
• NCBI Protein Information: E3 ubiquitin-protein ligase RNF34
• UniProt Protein Name: E3 ubiquitin-protein ligase RNF34
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: RNF34
• UniProt Synonym Gene Names: hRFI
• UniProt Entry Name: RNF34_HUMAN

NCBI Description

The protein encoded by this gene contains a RINF finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein interacts with DNAJA3/hTid-1, which is a DnaJ protein reported to function as a modulator of apoptosis. Overexpression of this gene in Hela cells was shown to confer the resistance to TNF-alpha induced apoptosis, suggesting an anti-apoptotic function of this protein. This protein can be cleaved by caspase-3 during the induction of apoptosis. This protein also targets p53 and phospho-p53 for degradation. Alternatively splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2012]

Uniprot Description

RNF34: Has E3 ubiquitin-protein ligase activity. Regulates the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. Protects cells against apoptosis induced by TNF. Binds phosphatidylinositol 5-phosphate and phosphatidylinositol 3- phosphate. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin ligase; Ligase; Ubiquitin conjugating system; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: cytoplasm; cytosol; endomembrane system; nuclear speck; nucleoplasm; nucleus; plasma membrane

Molecular Function: ligase activity; p53 binding; protein binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; zinc ion binding

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; protein ubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process