Rabbit RNF213 Polyclonal Antibody | anti-RNF213 antibody

RNF213 Polyclonal Antibody

Cat. Num. AAA3005945

• Gene Names: RNF213; ALO17; MYMY2; MYSTR; NET57; C17orf27; KIAA1618
• Reactivity: Human, Mouse, Rat, Pig, Cow, Horse, Sheep
• Applications: Immunohistochemistry
• Purity: affinity purified by Protein A
• Synonyms: RNF213; Polyclonal Antibody; RNF213 Polyclonal Antibody; C17orf27; ALK lymphoma oligomerization partner on chromosome 17; ALO17_HUMAN; Chromosome 17 open reading frame 27; NET57; Protein ALO17; Q63HN8; RING finger protein 213; RNF 213; anti-RNF213 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Pig, Cow, Horse, Sheep
• Clonality: Polyclonal
• Specificity: RNF213 Polyclonal Antibody detects endogenous levels of RNF213 protein.
• Purity/Purification: affinity purified by Protein A
• Form/Format: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• Applicable Applications for anti-RNF213 antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:100-500
• Immunogen: KLH conjugated synthetic peptide derived from human RNF213:4701-4800/5207
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RNF213) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C.)

Immunohistochemistry (IHC)

(Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RNF213) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C.)

Immunohistochemistry (IHC)

(Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RNF213) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C.)

Related Product Information for anti-RNF213 antibody

The RING-type zinc finger motif is present in a number of viral and eukaryotic proteins and is made up of a conserved cysteine-rich domain that is able to bind two zinc atoms. Proteins that contain this conserved domain are generally involved in the ubiquitination pathway of protein degradation. RNF213 (ring finger protein 213), also known as NET57, is a 3,280 amino acid protein containing one RING-type zinc finger domain through which it may play a role in transcriptional regulation and protein degradation. RNF213 is encoded by a gene located on human chromosome 17, which comprises over 2.5% of the human genome and encodes over 1,200 genes. Two key tumor suppressor genes are associated with chromosome 17, namely, p53 and BRCA1. Tumor suppressor p53 is necessary for maintenance of cellular genetic integrity by moderating cell fate through DNA repair versus cell death. Malfunction or loss of p53 expression is associated with malignant cell growth and Li-Fraumeni syndrome. Like p53, BRCA1 is directly involved in DNA repair, though specifically it is recognized as a genetic determinant of early onset breast cancer and predisposition to cancers of the ovary, colon, prostate gland and fallopian tubes.

NCBI and Uniprot Product Information

• NCBI GI #: 597709777
• NCBI GeneID: 57674
• NCBI Accession #: NP_001243000.2
• NCBI GenBank Nucleotide #: NM_001256071.2
• UniProt Accession #: Q9HCF4; Q658P6; Q69YK7; Q6MZR1; Q8IWF4; Q8IZX1; Q8IZX2; Q8N406; Q8TEU0; C9JCP4; D6RI12; F8WKS1; Q63HN8
• Molecular Weight: 64,260 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase RNF213 isoform 3
• NCBI Official Synonym Full Names: ring finger protein 213
• NCBI Official Symbol: RNF213
• NCBI Official Synonym Symbols: ALO17; MYMY2; MYSTR; NET57; C17orf27; KIAA1618
• NCBI Protein Information: E3 ubiquitin-protein ligase RNF213; mysterin; ALK lymphoma oligomerization partner on chromosome 17
• UniProt Protein Name: E3 ubiquitin-protein ligase RNF213
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: RNF213

NCBI Description

This gene encodes a protein containing a C3HC4-type RING finger domain, which is a specialized type of Zn-finger that binds two atoms of zinc and is thought to be involved in mediating protein-protein interactions. The protein also contains an AAA domain, which is associated with ATPase activity. This gene is a susceptibility gene for Moyamoya disease, a vascular disorder of intracranial arteries. This gene is also a translocation partner in anaplastic large cell lymphoma and inflammatory myofibroblastic tumor cases, where a t(2;17)(p23;q25) translocation has been identified with the anaplastic lymphoma kinase (ALK) gene on chromosome 2, and a t(8;17)(q24;q25) translocation has been identified with the MYC gene on chromosome 8. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]

Uniprot Description

RNF213 iso5: Probable E3 ubiquitin-protein ligase that may play a role in angiogenesis. May also have an ATPase activity. Defects in RNF213 are the cause of susceptibility to Moyamoya disease type 2 (MYMY2). A progressive cerebral angiopathy characterized by bilateral intracranial carotid artery stenosis and telangiectatic vessels in the region of the basal ganglia. The abnormal vessels resemble a 'puff of smoke' (moyamoya) on cerebral angiogram. Affected individuals can develop transient ischemic attacks and/or cerebral infarction, and rupture of the collateral vessels can cause intracranial hemorrhage. Hemiplegia of sudden onset and epileptic seizures constitute the prevailing presentation in childhood, while subarachnoid bleeding occurs more frequently in adults. A chromosomal aberration involving ALO17 is associated with anaplastic large-cell lymphoma (ALCL). Translocation t(2;17)(p23;q25) with ALK. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.-; Oncoprotein; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: cytoplasm; cytosol; membrane; nucleolus

Molecular Function: ATPase activity; metal ion binding; ubiquitin-protein transferase activity

Biological Process: angiogenesis; negative regulation of non-canonical Wnt signaling pathway; protein autoubiquitination; protein homooligomerization; protein polyubiquitination; protein ubiquitination; sprouting angiogenesis; ubiquitin-dependent protein catabolic process

Disease: Moyamoya Disease 2